Frequent microsatellite alterations of chromosome locus 4q13.1 in oral squamous cell carcinomas

Shu Chun Lin, Mei Fong Chang, Ming Yi Chung, Che Shoa Chang, Shou Yen Kao, Chung Ji Liu, Kuo Wei Chang*


研究成果: Article同行評審

13 引文 斯高帕斯(Scopus)


BACKGROUND: Studies have revealed that losses of chromosome 4q24-25 regions are frequent in cancers including head and neck squamous cell carcinoma. Our previous comparative genomic hybridization analysis showed extensive losses of chromosome arm 4q in oral squamous cell carcinoma (OSCC). METHODS: To be more precise in mapping the potential regions of allelic losses and to understand the microsatellite instability (MSI) on 4q involving in oral pathogenesis, we performed allelotypings using eight polymorphic markers. Microsatellite analyses were first performed on 100 randomly selected controls to confirm the high informative rates of markers. Twenty OSCC tissues were microdissected from surgical specimens for microsatellite alterations (MA) analysis. RESULTS: HA was observed in 95% OSCC cases. The most eminently altered locus was 4q13.1 (75%), followed by 4q22.2 and 4q32.1 (55%). Allelic losses also occurred most frequently on these loci. Thirty-five percent cases had MA spanning 4q13.1 to 4q21.1. HSI occurred in 35% OSCC, at a lesser extent compared with allelic losses. The most common locus for MSI was 4q2l.2 (20%). In addition, 4q MSI was significantly associated with the lymph node metastasis of OSCC (P = 0.01). So far, most tumor suppressor genes on 4q have not been specified. CONCLUSION: Our results were additive to previous findings and proposed novel scenario of suppressor loci located at 4q13.1-21.1 whose inactivation could be important for progression of OSCC.

頁(從 - 到)209-213
期刊Journal of Oral Pathology and Medicine
出版狀態Published - 4月 2005


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