Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy

Aaron D. Slepkov, Andrew Ridsdale, Huei Ning Wan, Ming Hao Wang, Adrian F. Pegoraro, Douglas J. Moffatt, John P. Pezacki, Fu Jen Kao, Albert Stolow

研究成果: Article同行評審

20 引文 斯高帕斯(Scopus)

摘要

We demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells.

原文English
文章編號021103
期刊Journal of Biomedical Optics
16
發行號2
DOIs
出版狀態Published - 2月 2011

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