@article{040595fe75fb47b99a1387f610aa6cb7,
title = "Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy",
abstract = "We demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells.",
keywords = "Raman spectroscopy, fiber optic applications, laser-induced fluorescence, nonlinear optics, scanning microscopy",
author = "Slepkov, {Aaron D.} and Andrew Ridsdale and Wan, {Huei Ning} and Wang, {Ming Hao} and Pegoraro, {Adrian F.} and Moffatt, {Douglas J.} and Pezacki, {John P.} and Kao, {Fu Jen} and Albert Stolow",
note = "Funding Information: This research was largely funded by a joint NRC-NSC-ITRI Canada-Taiwan Cooperation project “Digital FLIM-CARS Microscopy.” We would like to thank Rodney Lyn for his expertise and time in HuH-7 cell sample preparation and general discussion. We gratefully acknowledge Guillaume Labroille for fruitful discussions and for his input into optical alignment and layout design.",
year = "2011",
month = feb,
doi = "10.1117/1.3490641",
language = "English",
volume = "16",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "2",
}