TY - JOUR
T1 - Fluorescence lifetime-resolved imaging
AU - Chen, Yi-Chun
AU - Clegg, Robert M.
PY - 2009/11
Y1 - 2009/11
N2 - This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the fundamentals of the measured fluorescence response, and introduce the basic measurement methodologies. We also emphasize the complex nature of the fluorescence response in FLI signals, and introduce certain analysis methods that are appropriate and informative for complex fluorescence decays. The advantages of model independent analyses are discussed and examples given.
AB - This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the fundamentals of the measured fluorescence response, and introduce the basic measurement methodologies. We also emphasize the complex nature of the fluorescence response in FLI signals, and introduce certain analysis methods that are appropriate and informative for complex fluorescence decays. The advantages of model independent analyses are discussed and examples given.
KW - Fluorescence lifetime-resolved imaging
KW - Fluorescence lifetime-resolved imaging microscopy
KW - Frequency domain
KW - Microscopy
KW - Model independent analysis
KW - Spectral FLIM
KW - Time-domain
UR - http://www.scopus.com/inward/record.url?scp=76149090164&partnerID=8YFLogxK
U2 - 10.1007/s11120-009-9458-7
DO - 10.1007/s11120-009-9458-7
M3 - Review article
C2 - 19763875
AN - SCOPUS:76149090164
SN - 0166-8595
VL - 102
SP - 143
EP - 155
JO - Photosynthesis Research
JF - Photosynthesis Research
IS - 2
ER -