Fluorescence lifetime imaging microscopy with subdiffraction-limited resolution

Po Yen Lin*, Yi Cheng Lin, Chia Seng Chang, Fu Jen Kao

*此作品的通信作者

研究成果: Article同行評審

18 引文 斯高帕斯(Scopus)

摘要

In this study, we demonstrate subdiffraction-limited fluorescence lifetime imaging microscopy (FLIM) by engineering the point spread function (PSF) with stimulated emission depletion (STED). The enhanced spatial resolution allows the number of fluorophores in the PSF to reduce in turn the associated heterogeneity in lifetime analysis. Moreover, time gating can be performed using time-correlated single photon counting (TCSPC) to carefully select detected fluorescence photons so as to optimize the spatial resolution and the signal-to-noise ratio in STED imaging. This flexibility also supports the removal of the unintended effects of lifetime reduction that is caused by STED pulses.

原文English
文章編號028004
期刊Japanese journal of applied physics
52
發行號2
DOIs
出版狀態Published - 2月 2013

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