Fluorescence lifetime imaging comes of age how to do it and how to interpret it

Yi-Chun Chen, Bryan Q. Spring, Robert M. Clegg*

*此作品的通信作者

研究成果: Chapter同行評審

8 引文 斯高帕斯(Scopus)

摘要

Fluorescence lifetime imaging (FLI) has been used widely for measuring biomedical samples. Practical guidelines on taking successful FLI data are provided to avoid common errors that arise during the measurement. Several methods for analyzing and interpreting FLI results are also introduced; e.g., a model-free data analysis method called the polar plot allows visualization and analysis of FLI data without iterative fitting, and an image denoising algorithm called variance-stabilizing-transform TI Haar helps to elucidate the information of a complex biomedical sample. The instrument considerations and data analysis of Spectral-FLI are also discussed.

原文English
主出版物標題Spectroscopic Methods of Analysis
主出版物子標題Methods and Protocols
發行者Humana Press Inc.
頁面1-22
頁數22
ISBN(列印)9781617798054
DOIs
出版狀態Published - 1 1月 2012

出版系列

名字Methods in Molecular Biology
875
ISSN(列印)1064-3745

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