Femtosecond laser-induced cleaving of protein crystal in water solution

Masafumi Kashii, Yoichiroh Hosokawa*, Hiroshi Kitano, Hiroaki Adachi, Yusuke Mori, Kazufumi Takano, Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami, Kazuomi Sugamoto, Hideki Yoshikawa, Takatomo Sasaki, Hiroshi Masuhara

*此作品的通信作者

研究成果: Article同行評審

9 引文 斯高帕斯(Scopus)

摘要

For precise X-ray diffraction (XRD) measurement giving the three-dimensional structure of proteins, it is important to prepare high-quality single crystals with suitable shape. As a new processing technique to obtain such protein crystals, we employed femtosecond laser-induced cleaving of protein crystal in a growth vessel containing water solution. An intact protein crystal was precisely processed without mechanical contact in its sealed growth vessel by focusing femtosecond laser pulses. We confirmed that three-dimensional processing of the crystal in its supersaturated solution was realized using multiphoton absorption and that the processing was efficiently enhanced by the cleaving behavior attributed to a photomechanical mechanism of the femtosecond laser ablation.

原文English
頁(從 - 到)6447-6450
頁數4
期刊Applied Surface Science
253
發行號15
DOIs
出版狀態Published - 30 五月 2007

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