Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

Pei Yu Chang; Kuo Wei Chang; Ciou Wen Lin; Po Chen Shih; Cheng Hsien Liu; Wen-Syang Hsu; Shih Kang Fan; Chin Jung Li; Chang Hung Tien; Hong Yuan Huang; Da Jeng Yao

doi:10.1109/NANOMED.2012.6509134

Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

Pei Yu Chang, Kuo Wei Chang, Ciou Wen Lin, Po Chen Shih, Cheng Hsien Liu, Wen-Syang Hsu, Shih Kang Fan, Chin Jung Li, Chang Hung Tien, Hong Yuan Huang, Da Jeng Yao

機械工程學系

研究成果: Conference contribution › 同行評審

摘要

Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.

原文	English
主出版物標題	NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
頁面	32-35
頁數	4
DOIs	https://doi.org/10.1109/NANOMED.2012.6509134
出版狀態	Published - 1 12月 2012
事件	6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, 泰國持續時間: 4 11月 2012 → 7 11月 2012

出版系列

名字	IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN（列印）	2159-6964
ISSN（電子）	2159-6972

Conference

Conference	6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
國家/地區	泰國
城市	Bangkok
期間	4/11/12 → 7/11/12

存取文件

10.1109/NANOMED.2012.6509134

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Link to publication in Scopus

引用此

Chang, P. Y., Chang, K. W., Lin, C. W., Shih, P. C., Liu, C. H., Hsu, W.-S., Fan, S. K., Li, C. J., Tien, C. H., Huang, H. Y., & Yao, D. J. (2012). Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. 於 NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (頁 32-35). 文章 6509134 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). https://doi.org/10.1109/NANOMED.2012.6509134

Chang, Pei Yu ; Chang, Kuo Wei ; Lin, Ciou Wen 等. / Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering. 2012. 頁 32-35 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED).

@inproceedings{f996becde56c411899273b4b4a4938e1,

title = "Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells",

abstract = "Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.",

keywords = "3D chip, Mimetic uterus, Thin membrane",

author = "Chang, {Pei Yu} and Chang, {Kuo Wei} and Lin, {Ciou Wen} and Shih, {Po Chen} and Liu, {Cheng Hsien} and Wen-Syang Hsu and Fan, {Shih Kang} and Li, {Chin Jung} and Tien, {Chang Hung} and Huang, {Hong Yuan} and Yao, {Da Jeng}",

year = "2012",

month = dec,

day = "1",

doi = "10.1109/NANOMED.2012.6509134",

language = "English",

isbn = "9781467351027",

series = "IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED",

pages = "32--35",

booktitle = "NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering",

note = "6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 ; Conference date: 04-11-2012 Through 07-11-2012",

}

Chang, PY, Chang, KW, Lin, CW, Shih, PC, Liu, CH, Hsu, W-S, Fan, SK, Li, CJ, Tien, CH, Huang, HY & Yao, DJ 2012, Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. 於 NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering., 6509134, IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED, 頁 32-35, 6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012, Bangkok, 泰國, 4/11/12. https://doi.org/10.1109/NANOMED.2012.6509134

Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. / Chang, Pei Yu; Chang, Kuo Wei; Lin, Ciou Wen 等.
NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering. 2012. p. 32-35 6509134 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED).

研究成果: Conference contribution › 同行評審

TY - GEN

T1 - Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

AU - Chang, Pei Yu

AU - Chang, Kuo Wei

AU - Lin, Ciou Wen

AU - Shih, Po Chen

AU - Liu, Cheng Hsien

AU - Hsu, Wen-Syang

AU - Fan, Shih Kang

AU - Li, Chin Jung

AU - Tien, Chang Hung

AU - Huang, Hong Yuan

AU - Yao, Da Jeng

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.

AB - Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.

KW - 3D chip

KW - Mimetic uterus

KW - Thin membrane

UR - http://www.scopus.com/inward/record.url?scp=84880194599&partnerID=8YFLogxK

U2 - 10.1109/NANOMED.2012.6509134

DO - 10.1109/NANOMED.2012.6509134

M3 - Conference contribution

AN - SCOPUS:84880194599

SN - 9781467351027

T3 - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED

SP - 32

EP - 35

BT - NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering

T2 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012

Y2 - 4 November 2012 through 7 November 2012

ER -

Chang PY, Chang KW, Lin CW, Shih PC, Liu CH, Hsu WS 等. Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells. 於 NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering. 2012. p. 32-35. 6509134. (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). doi: 10.1109/NANOMED.2012.6509134

Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

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