Expression and secretion of hepatitis B viral surface antigen in E. coli

Yan-Hwa Wu Lee; Yao Tsung Tung; Szecheng J. Lo

doi:10.1016/0006-291X(86)91033-8

Expression and secretion of hepatitis B viral surface antigen in E. coli

Yan-Hwa Wu Lee^*, Yao Tsung Tung, Szecheng J. Lo

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生物科技學系

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5 引文斯高帕斯（Scopus）

摘要

Hepatitis B viral surface antigen (HBsAg) gene was subcloned into the BglII site of Bacillus licheniformis penicillinase (penP) gene of secretory vector pJP104. Expression and secretion of HBsAg protein was achieved by the E. coli CS412 carrying the plasmid pJPS2 in which the penP:HBsAg hybrid gene was under the control of two promoters, lipoprotein (lpp) and penP, spaced 450 bases apart. The secreted form of HBsAg encoded by the hybrid penP:HBsAg gene of plasmid pJPS2 was purified by immunoaffinity chromatography and found to be a 25 kilodalton protein.

原文	English
頁（從 - 到）	1042-1049
頁數	8
期刊	Biochemical and Biophysical Research Communications
卷	135
發行號	3
DOIs	https://doi.org/10.1016/0006-291X(86)91033-8
出版狀態	Published - 28 3月 1986

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title = "Expression and secretion of hepatitis B viral surface antigen in E. coli",

abstract = "Hepatitis B viral surface antigen (HBsAg) gene was subcloned into the BglII site of Bacillus licheniformis penicillinase (penP) gene of secretory vector pJP104. Expression and secretion of HBsAg protein was achieved by the E. coli CS412 carrying the plasmid pJPS2 in which the penP:HBsAg hybrid gene was under the control of two promoters, lipoprotein (lpp) and penP, spaced 450 bases apart. The secreted form of HBsAg encoded by the hybrid penP:HBsAg gene of plasmid pJPS2 was purified by immunoaffinity chromatography and found to be a 25 kilodalton protein.",

author = "{Wu Lee}, Yan-Hwa and Tung, {Yao Tsung} and Lo, {Szecheng J.}",

year = "1986",

month = mar,

day = "28",

doi = "10.1016/0006-291X(86)91033-8",

language = "English",

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T1 - Expression and secretion of hepatitis B viral surface antigen in E. coli

AU - Wu Lee, Yan-Hwa

AU - Tung, Yao Tsung

AU - Lo, Szecheng J.

PY - 1986/3/28

Y1 - 1986/3/28

N2 - Hepatitis B viral surface antigen (HBsAg) gene was subcloned into the BglII site of Bacillus licheniformis penicillinase (penP) gene of secretory vector pJP104. Expression and secretion of HBsAg protein was achieved by the E. coli CS412 carrying the plasmid pJPS2 in which the penP:HBsAg hybrid gene was under the control of two promoters, lipoprotein (lpp) and penP, spaced 450 bases apart. The secreted form of HBsAg encoded by the hybrid penP:HBsAg gene of plasmid pJPS2 was purified by immunoaffinity chromatography and found to be a 25 kilodalton protein.

AB - Hepatitis B viral surface antigen (HBsAg) gene was subcloned into the BglII site of Bacillus licheniformis penicillinase (penP) gene of secretory vector pJP104. Expression and secretion of HBsAg protein was achieved by the E. coli CS412 carrying the plasmid pJPS2 in which the penP:HBsAg hybrid gene was under the control of two promoters, lipoprotein (lpp) and penP, spaced 450 bases apart. The secreted form of HBsAg encoded by the hybrid penP:HBsAg gene of plasmid pJPS2 was purified by immunoaffinity chromatography and found to be a 25 kilodalton protein.

UR - http://www.scopus.com/inward/record.url?scp=0022485752&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(86)91033-8

DO - 10.1016/0006-291X(86)91033-8

M3 - Article

C2 - 3516144

AN - SCOPUS:0022485752

SN - 0006-291X

VL - 135

SP - 1042

EP - 1049

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Expression and secretion of hepatitis B viral surface antigen in E. coli

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