This study to explore the eye movement patterns with underlying cognitive process of optic reasoning between science and non-science major students that have different prior knowledge of optical concepts. There are 33 science major and 33 non-science major undergraduate students were involved in this study. The results showed the science major students and non-science major students have improved learning performance, and science major students significantly outperformed than non-science major students in pre-test and post-test. In addition, the eye movement patterns indicated the mean fixation duration and mean regression duration within areas of interest (AOIs) of pictorial and text region were longer in science major students than that in non-science major students. To get the learning performance and eye movement patterns, we suggested that science major students involved deeper cognitive processing and greater attention during the optic reasoning learning content and might be reflected on eye movement patterns.