This paper presents experimental investigations of passive mixing in a microfluidic channel with different zigzag angles. Zigzag channel is commonly used for microfluidic mixing because it does not need an additional control unit and can be easily implemented in a lab-on-a-chip system. In this work, microfluidic channels with six different zigzag angles, from θ = 0° to θ = 75°, are tested under ten different flow rates corresponding to Reynolds number from 0.309 to 309. Two colored liquids are mixed with the zigzag channels and mixing performance is evaluated based on the color of the pixels on the region of interest from captured images. According to the results, we found that the mixing performance is almost independent of the zigzag angle in the low-speed regime where its Reynolds number is less than 4. The mixing became very much depending on the zigzag angle in the high-speed regime where its Reynolds number is greater than 100. Microfluidic mixing is needed for Lab-on-a-chip applications in both low flow speed, such as medium perfusion for cell culture, and high flow speed, such as high-speed sensing on a point-of-care device. This work is aimed to provide practical information on zigzag mixing for chip design and applications.