Evaluation of Mitochondria Content and Function in Live Cells by Multicolor Flow Cytometric Analysis

Hsiu Han Fan, Tsung Lin Tsai, Ivan L. Dzhagalov, Chia Lin Hsu*

*此作品的通信作者

研究成果: Chapter同行評審

4 引文 斯高帕斯(Scopus)

摘要

To evaluate how a cell responds to the external stimuli, treatment, or alteration of the microenvironment, the quantity and quality of mitochondria are commonly used as readouts. However, it is challenging to apply mitochondrial analysis to the samples that are composed of mixed cell populations originating from tissues or when multiple cell populations are of interest, using methods such as Western blot, electron microscopy, or extracellular flux analysis. Flow cytometry is a technique allowing the detection of individual cell status and its identity simultaneously when used in combination with surface markers. Here we describe how to combine mitochondria-specific dyes or the dyes targeting the superoxide produced by mitochondria with surface marker staining to measure the mitochondrial content and activity in live cells by flow cytometry. This method can be applied to all types of cells in suspension and is particularly useful for analysis of samples composed of heterogeneous cell populations.

原文English
主出版物標題Methods in Molecular Biology
發行者Humana Press Inc.
頁面203-213
頁數11
DOIs
出版狀態Published - 6月 2021

出版系列

名字Methods in Molecular Biology
2276
ISSN(列印)1064-3745
ISSN(電子)1940-6029

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