Evaluation of dry stored disposable sensor strip on rapid SARS-CoV-2 detection platform

Chao Ching Chiang, Chan Wen Chiu, Fan Ren, Cheng Tse Tsai, Yu Te Liao, Josephine F. Esquivel-Upshaw, Stephen J. Pearton

研究成果: Article同行評審

摘要

This study investigated a SARS-CoV-2 virus detection mechanism using dry-stored disposable strips. The accuracy of this sensing platform is as good as polymerase chain reaction (PCR) with a detection time of fewer than 30 s. SARS-CoV-2 antibodies were biofunctionalized on disposable strips similar to glucose detection strips to detect the presence and concentrations of SARS-CoV-2 in saliva samples. Eight 1 ms electric pulses were sent through the sensor strip with a saliva sample in its microfluidic chamber. A circuit board embedded with MOSFET was also employed to amplify the detected signals and convert the signal to digital readings displayed on an LCD screen. The COV-antibody functionalized disposable strips were stored in a dry condition for at least one day before analyzing clinical human saliva samples with known cycling threshold (Ct) values confirmed with conventional PCR tests. Results demonstrate our system is capable of showing qualitative positive or negative results within 30 s and providing quantitative SARS-CoV-2 concentrations in terms of Ct values in 5 min.

原文English
文章編號012204
期刊Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics
41
發行號1
DOIs
出版狀態Published - 1月 2023

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