TY - JOUR
T1 - Evaluation of different analysis and identification methods for Salmonella detection in surface drinking water sources
AU - Hsu, Bing Mu
AU - Huang, Kuan Hao
AU - Huang, Shih Wei
AU - Tseng, Kuo Chih
AU - Su, Ming Jen
AU - Lin, Wei Chen
AU - Ji, Dar Der
AU - Shih, Feng Cheng
AU - Chen, Jyh Larng
AU - Kao, Po Min
N1 - Funding Information:
This work was supported by a research grant from National Science Council of Taiwan, ROC ( NSC97-2221-E-194-006-MY3 ). We are grateful the editing of Wallace Academic Editing in Taiwan and grateful the experimental work by Dr. Chien-Shun Chiou and Mrs. Yo-Wen Wang from the third branch office, Centers for Disease Control, Taiwan, ROC.
PY - 2011/9/15
Y1 - 2011/9/15
N2 - The standard method for detecting Salmonella generally analyzes food or fecal samples. Salmonella often occur in relatively low concentrations in environmental waters. Therefore, some form of concentration and proliferation may be needed. This study compares three Salmonella analysis methods and develops a new Salmonella detection procedure for use in environmental water samples. The new procedure for Salmonella detection include water concentration, nutrient broth enrichment, selection of Salmonella containing broth by PCR, isolation of Salmonella strains by selective culture plates, detection of possible Salmonella isolate by PCR, and biochemical testing. Serological assay and pulsed-field gel electrophoresis (PFGE) can be used to identify Salmonella serotype and genotype, respectively. This study analyzed 116 raw water samples taken from 18 water plants and belonging to 5 watersheds. Of these 116, 10 water samples (8.6%) taken from 7 water plants and belonging to 4 watersheds were positive for a Salmonella-specific polymerase chain reaction targeting the invA gene. Guided by serological assay results, this study identified 7 cultured Salmonella isolates as Salmonella enterica serovar: Alnaby, Enteritidis, Houten, Montevideo, Newport, Paratyphi B var. Java, and Victoria. These seven Salmonella serovars were identified in clinical cases for the same geographical areas, but only one of them was 100% homologous with clinical cases in the PFGE pattern.
AB - The standard method for detecting Salmonella generally analyzes food or fecal samples. Salmonella often occur in relatively low concentrations in environmental waters. Therefore, some form of concentration and proliferation may be needed. This study compares three Salmonella analysis methods and develops a new Salmonella detection procedure for use in environmental water samples. The new procedure for Salmonella detection include water concentration, nutrient broth enrichment, selection of Salmonella containing broth by PCR, isolation of Salmonella strains by selective culture plates, detection of possible Salmonella isolate by PCR, and biochemical testing. Serological assay and pulsed-field gel electrophoresis (PFGE) can be used to identify Salmonella serotype and genotype, respectively. This study analyzed 116 raw water samples taken from 18 water plants and belonging to 5 watersheds. Of these 116, 10 water samples (8.6%) taken from 7 water plants and belonging to 4 watersheds were positive for a Salmonella-specific polymerase chain reaction targeting the invA gene. Guided by serological assay results, this study identified 7 cultured Salmonella isolates as Salmonella enterica serovar: Alnaby, Enteritidis, Houten, Montevideo, Newport, Paratyphi B var. Java, and Victoria. These seven Salmonella serovars were identified in clinical cases for the same geographical areas, but only one of them was 100% homologous with clinical cases in the PFGE pattern.
KW - PFGE
KW - Salmonella
KW - Salmonella isolation
KW - Water safety
UR - http://www.scopus.com/inward/record.url?scp=80052045716&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2011.05.052
DO - 10.1016/j.scitotenv.2011.05.052
M3 - Article
C2 - 21782212
AN - SCOPUS:80052045716
SN - 0048-9697
VL - 409
SP - 4435
EP - 4441
JO - Science of the Total Environment
JF - Science of the Total Environment
IS - 20
ER -