Epigenetic regulation of WNT3A enhancer during regeneration of injured cortical neurons

Chu Yuan Chang; Jui Hung Hung; Liang Wei Huang; Joye Li; Ka Shing Fung; Cheng Fu Kao; Linyi Chen

doi:10.3390/ijms21051891

Epigenetic regulation of WNT3A enhancer during regeneration of injured cortical neurons

Chu Yuan Chang, Jui Hung Hung, Liang Wei Huang, Joye Li, Ka Shing Fung, Cheng Fu Kao, Linyi Chen^*

^*此作品的通信作者

數據科學與工程研究所

研究成果: Article › 同行評審

3 引文斯高帕斯（Scopus）

摘要

Traumatic brain injury is known to reprogram the epigenome. Chromatin immunoprecipitation-sequencing of histone H3 lysine 27 acetylation (H3K27ac) and tri-methylation of histone H3 at lysine 4 (H3K4me3) marks was performed to address the transcriptional regulation of candidate regeneration-associated genes. In this study, we identify a novel enhancer region for induced WNT3A transcription during regeneration of injured cortical neurons. We further demonstrated an increased mono-methylation of histone H3 at lysine 4 (H3K4me1) modification at this enhancer concomitant with a topological interaction between sub-regions of this enhancer and with promoter of WNT3A gene. Together, this study reports a novel mechanism for WNT3A gene transcription and reveals a potential therapeutic intervention for neuronal regeneration.

原文	English
文章編號	1891
頁數	16
期刊	International journal of molecular sciences
卷	21
發行號	5
DOIs	https://doi.org/10.3390/ijms21051891
出版狀態	Published - 10 3月 2020

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title = "Epigenetic regulation of WNT3A enhancer during regeneration of injured cortical neurons",

abstract = "Traumatic brain injury is known to reprogram the epigenome. Chromatin immunoprecipitation-sequencing of histone H3 lysine 27 acetylation (H3K27ac) and tri-methylation of histone H3 at lysine 4 (H3K4me3) marks was performed to address the transcriptional regulation of candidate regeneration-associated genes. In this study, we identify a novel enhancer region for induced WNT3A transcription during regeneration of injured cortical neurons. We further demonstrated an increased mono-methylation of histone H3 at lysine 4 (H3K4me1) modification at this enhancer concomitant with a topological interaction between sub-regions of this enhancer and with promoter of WNT3A gene. Together, this study reports a novel mechanism for WNT3A gene transcription and reveals a potential therapeutic intervention for neuronal regeneration.",

keywords = "Enhancer regulation, Epigenome, Histone modification, Neuronal regeneration, Transcriptional regulation, WNT3A",

author = "Chang, {Chu Yuan} and Hung, {Jui Hung} and Huang, {Liang Wei} and Joye Li and Fung, {Ka Shing} and Kao, {Cheng Fu} and Linyi Chen",

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AU - Chang, Chu Yuan

AU - Hung, Jui Hung

AU - Huang, Liang Wei

AU - Li, Joye

AU - Fung, Ka Shing

AU - Kao, Cheng Fu

AU - Chen, Linyi

PY - 2020/3/10

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KW - Enhancer regulation

KW - Epigenome

KW - Histone modification

KW - Neuronal regeneration

KW - Transcriptional regulation

KW - WNT3A

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Epigenetic regulation of WNT3A enhancer during regeneration of injured cortical neurons

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