Enhancing the sensitivity of localized surface plasmon resonance (LSPR) biosensors using nanorods and DNA aptamers

Po Chun Chuang, Pei Chen Liao, Yih Fan Chen*

*此作品的通信作者

研究成果: Conference contribution同行評審

5 引文 斯高帕斯(Scopus)

摘要

Localized surface plasmon resonance (LSPR) biosensors have drawn much attention for their promising application in point-of-care diagnostics. While surface plasmon resonance (SPR) biosensing systems have been well developed, LSPR systems have the advantages of simpler and more compact setups. The LSPR peak shifts caused by the binding of molecules to the LSPR substrates, however, are usually smaller than 1 nm if no signal amplification mechanism is used. When using nanoparticles to enhance the sensitivity of LSPR biosensors, because of the short field penetration depth, the nanoparticles should be very close to the LSPR substrate to induce significant shifts in the LSPR peak position. In this study, we used DNA aptamers and gold nanorods to significantly increase the change in the LSPR peak position with the concentration of the target molecules. We have successfully used the proposed mechanism to detect 0.1 nM interferon-gamma (IFN-γ), a biomarker related to the diagnosis of latent tuberculosis infection. The calibration curves obtained in pure buffers and serum-containing buffers show that accurate detection can be achieved even when the sample is from complex biological fluids such as serum. Because of the enhancement in the sensitivity by the proposed sensing scheme, it is possible to use a low-cost spectrometer to build a LSPR biosensing system.

原文English
主出版物標題Plasmonics in Biology and Medicine XII
編輯Joseph R. Lakowicz, Tuan Vo-Dinh
發行者SPIE
ISBN(電子)9781628414301
DOIs
出版狀態Published - 2015
事件Plasmonics in Biology and Medicine XII - San Francisco, United States
持續時間: 7 2月 20158 2月 2015

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
9340
ISSN(列印)1605-7422

Conference

ConferencePlasmonics in Biology and Medicine XII
國家/地區United States
城市San Francisco
期間7/02/158/02/15

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