Enhancement of gene therapy on hepatocellular carcinoma by sonoporation-parameter studies

Kun Che Tsai*, Lih Hwa Hwang, Shu Jyuan Yang, Che Kang Liao, Win Li Lin, Ming Jium Shieh, Wen Shiang Chen

*此作品的通信作者

研究成果: Conference article同行評審

摘要

Ultrasound (US) is believed to be a novel and effective tool to locally deliver gene into target tumors. US can temporally change the permeability of cell membrane and thus enhance the delivery of naked DNA into cells. In this study, the gene transfection in tumor and muscle tissues was evaluated by changing the contrast agent concentrations and US exposure durations. The luciferase and IL-12 mixed with SonoVue® were injected intramuscularly (IM) or intratumorally (IT), followed by exposure to US of 20% in duty cycle and 2 W/cm2 in intensity. The serum levels of IL-12 were determined by ELISA. Experimental results showed that both local and systemic expressions of the naked DNA in muscle are significantly higher than those in subcutaneous and liver tumors, and an order of muscle > subcutaneous tissue > liver tumor was found. Best transfection efficiency could be obtained by using 50% SonoVue in tumor and 30% in muscle after 10 min US exposure.

原文English
文章編號4803371
頁(從 - 到)562-565
頁數4
期刊Proceedings of the IEEE Ultrasonics Symposium
DOIs
出版狀態Published - 2008
事件2008 IEEE International Ultrasonics Symposium, IUS 2008 - Beijing, China
持續時間: 2 11月 20085 11月 2008

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