@inproceedings{46ab285dd3984f4fbac9650067682f76,
title = "Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective",
abstract = "Glycine N-methyltransferase (GNMT) is the most abundant hepatic methyltransferase and plays important roles in regulating methyl group metabolism. In the central nervous system, GNMT expression is low and its function has not been revealed. The present study examines the effect of GNMT overexpression by adenovirus-mediated transfer in cortical mixed neuron-glial cultures. Infection of adenovirus encoding green fluorescence protein to cultures demonstrates high preference for non-neuronal cells. Optimal GNMT overexpression in cultures by adenoviral GNMT (Ad-GNMT) infection not only induces protein kinase C phosphorylation, but also increases neuronaloligodendroglial survival. Furthermore, these Ad-GNMT-infected cultures are significantly resistant to H2O2 toxicity and lipopolysaccharide stimulation. Conditioned media from Ad-GNMT-infected microglia also significantly enhance neuronal survival. Taken together, enhanced GNMT expression in mixed neuronal-glial cultures is neuroprotective, most likely mediated through non-neuronal cells.",
keywords = "Adenovirus, GNMT, Lipopolysaccharide, Neuron-glia interaction",
author = "Tsai, {May Jywan} and Chen, {Yi Ming Arthur} and Weng, {Ching Feng} and Liou, {Dann Ying} and Yang, {Hsin Chun} and Chen, {Chien Hung} and Liao, {Roanna I.Hsin} and Kuo, {Fu Shan} and Chiu, {Chiuan Wen} and Kuo, {Huai Sheng} and Huang, {Ming Chao} and Lin, {Yi Lo} and Lee, {Meng Jen} and Kuo, {Wen Chun} and Huang, {Wen Cheng} and Henrich Cheng",
year = "2010",
month = jun,
doi = "10.1111/j.1749-6632.2009.05169.x",
language = "English",
isbn = "9781573317771",
series = "Annals of the New York Academy of Sciences",
publisher = "Blackwell Publishing Inc.",
pages = "194--203",
booktitle = "Neuroprotective Agents",
}