Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping

Hsu Hua Yeh; Shu Lin Chang; Yi Ming Chiang; Kenneth S. Bruno; Berl R. Oakley; Tung-Kung Wu; Clay C.C. Wang

doi:10.1021/ol303328t

Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping

Hsu Hua Yeh, Shu Lin Chang, Yi Ming Chiang, Kenneth S. Bruno, Berl R. Oakley, Tung-Kung Wu^*, Clay C.C. Wang

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生物科技學系

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25 引文斯高帕斯（Scopus）

摘要

We reannotated the A. niger NR-PKS gene, e-gw1-19.204, and its downstream R domain gene, est-GWPlus-C-190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID-06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.

原文	English
頁（從 - 到）	756-759
頁數	4
期刊	Organic Letters
卷	15
發行號	4
DOIs	https://doi.org/10.1021/ol303328t
出版狀態	Published - 15 2月 2013

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10.1021/ol303328t

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abstract = "We reannotated the A. niger NR-PKS gene, e-gw1-19.204, and its downstream R domain gene, est-GWPlus-C-190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID-06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.",

author = "Yeh, {Hsu Hua} and Chang, {Shu Lin} and Chiang, {Yi Ming} and Bruno, {Kenneth S.} and Oakley, {Berl R.} and Tung-Kung Wu and Wang, {Clay C.C.}",

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T1 - Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping

AU - Yeh, Hsu Hua

AU - Chang, Shu Lin

AU - Chiang, Yi Ming

AU - Bruno, Kenneth S.

AU - Oakley, Berl R.

AU - Wu, Tung-Kung

AU - Wang, Clay C.C.

PY - 2013/2/15

Y1 - 2013/2/15

N2 - We reannotated the A. niger NR-PKS gene, e-gw1-19.204, and its downstream R domain gene, est-GWPlus-C-190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID-06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.

AB - We reannotated the A. niger NR-PKS gene, e-gw1-19.204, and its downstream R domain gene, est-GWPlus-C-190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID-06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.

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JO - Organic Letters

JF - Organic Letters

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Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping

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