Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis

Kai Wen Hsu, An Ming Wang, Yueh Hsin Ping, Kuo Hung Huang, Tzu Ting Huang, Hsin Chen Lee, Su Shun Lo, Chin Wen Chi, Tien Shun Yeh*


研究成果: Article同行評審

44 引文 斯高帕斯(Scopus)


Gastric carcinoma is one of the most common malignancies and the second most lethal cancer worldwide. The mechanisms underlying aggressiveness of gastric cancer still remain obscure. c-Myc promoter binding protein 1 (MBP-1) is a negative regulator of c-myc expression and ubiquitously expressed in normal human tissues. It is produced by alternative translation initiation of α-enolase gene. Both MBP-1 and α-enolase are involved in the control of tumorigenesis including gastric cancer. MicroRNAs (miRNAs) are involved in tumorigenesis and could have diagnostic, prognostic and therapeutic potential. In this study, whether miRNAs modulate tumorigenesis of gastric cancer cells through targeting MBP-1 was evaluated. We found that miR-363 targets 3′-untranslated region of human MBP-1/α-enolase messenger RNA. The exogenous miR-363 promotes growth, viability, progression, epithelial-mesenchymal transition and tumorsphere formation of SC-M1 gastric cancer cells through downregulation of MBP-1, whereas the knockdown of endogenous miR-363 suppresses tumorigenesis and progression of SC-M1 cells via upregulation of MBP-1. The miR-363/MBP-1 axis is also involved in the control of carcinogenesis in KATO III and SNU-16 gastric cancer cells. Furthermore, miR-363 induces the xenografted tumor growth and lung metastasis of SC-M1 cells through MBP-1 in vivo. Taken together, these results suggest that miR-363 plays an important role in the increment of gastric carcinogenesis via targeting MBP-1.

頁(從 - 到)208-217
出版狀態Published - 1月 2014


深入研究「Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis」主題。共同形成了獨特的指紋。