摘要
DNA strand and enzyme subunit specificities involved in the interaction between E. coli RNA polymerase and T7 DNA were studied by photo-crosslinking techniques. In non-specific enzyme-DNA complexes, subunits σ, β, and β′ were crosslinked to both strands of the DNA. Under conditions leading to specific enzyme-promoter complexes, however, only σ and β subunits were crosslinked. The σ subunit was crosslinked preferentially to the non-sense strand at promoter sites. No such strand specificity was observed for the β subunit. These results provide insight into the molecular mechanism of promoter recognition and indicate that the interaction between RNA polymerase and DNA template is different at promoters and at non-specific sites.
原文 | English |
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頁(從 - 到) | 5895-5912 |
頁數 | 18 |
期刊 | Nucleic acids research |
卷 | 8 |
發行號 | 23 |
DOIs | |
出版狀態 | Published - 11 12月 1980 |