DNA strand specificity in promoter recognition by RNA polymerase

Chan Suk Park, Zaharia Hillel, Cheng-Wen Wu*

*此作品的通信作者

研究成果: Article同行評審

24 引文 斯高帕斯(Scopus)

摘要

DNA strand and enzyme subunit specificities involved in the interaction between E. coli RNA polymerase and T7 DNA were studied by photo-crosslinking techniques. In non-specific enzyme-DNA complexes, subunits σ, β, and β′ were crosslinked to both strands of the DNA. Under conditions leading to specific enzyme-promoter complexes, however, only σ and β subunits were crosslinked. The σ subunit was crosslinked preferentially to the non-sense strand at promoter sites. No such strand specificity was observed for the β subunit. These results provide insight into the molecular mechanism of promoter recognition and indicate that the interaction between RNA polymerase and DNA template is different at promoters and at non-specific sites.

原文English
頁(從 - 到)5895-5912
頁數18
期刊Nucleic acids research
8
發行號23
DOIs
出版狀態Published - 11 12月 1980

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