Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements

Chia-Ching Chang; Po Yen Lin; Yen Fu Chen; Chia Seng Chang; Lou Sing Kan

doi:10.1063/1.2809406

Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements

Chia-Ching Chang^*, Po Yen Lin, Yen Fu Chen, Chia Seng Chang, Lou Sing Kan

^*此作品的通信作者

生物科技學系

研究成果: Article › 同行評審

7 引文斯高帕斯（Scopus）

摘要

We have detected the dynamics of 17-mer DNA triplex dissociation mechanism at the molecular level. Fluorescence resonance energy transfer (FRET) was used as an indicator of intermolecular interaction in nanometer range, whereas atomic force microscopy (AFM) was employed to address single molecule with sub-angstrom precision. The maximum rupture force of DNA triplex was found at pH 4.65, consistent with macroscopic observations. These results indicated that the FRET together with an AFM detection system could be used to reveal the DNA triplex interaction in nanometer scale unambiguously.

原文	English
文章編號	203901
期刊	Applied Physics Letters
卷	91
發行號	20
DOIs	https://doi.org/10.1063/1.2809406
出版狀態	Published - 12 11月 2007

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abstract = "We have detected the dynamics of 17-mer DNA triplex dissociation mechanism at the molecular level. Fluorescence resonance energy transfer (FRET) was used as an indicator of intermolecular interaction in nanometer range, whereas atomic force microscopy (AFM) was employed to address single molecule with sub-angstrom precision. The maximum rupture force of DNA triplex was found at pH 4.65, consistent with macroscopic observations. These results indicated that the FRET together with an AFM detection system could be used to reveal the DNA triplex interaction in nanometer scale unambiguously.",

author = "Chia-Ching Chang and Lin, {Po Yen} and Chen, {Yen Fu} and Chang, {Chia Seng} and Kan, {Lou Sing}",

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doi = "10.1063/1.2809406",

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T1 - Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements

AU - Chang, Chia-Ching

AU - Lin, Po Yen

AU - Chen, Yen Fu

AU - Chang, Chia Seng

AU - Kan, Lou Sing

PY - 2007/11/12

Y1 - 2007/11/12

N2 - We have detected the dynamics of 17-mer DNA triplex dissociation mechanism at the molecular level. Fluorescence resonance energy transfer (FRET) was used as an indicator of intermolecular interaction in nanometer range, whereas atomic force microscopy (AFM) was employed to address single molecule with sub-angstrom precision. The maximum rupture force of DNA triplex was found at pH 4.65, consistent with macroscopic observations. These results indicated that the FRET together with an AFM detection system could be used to reveal the DNA triplex interaction in nanometer scale unambiguously.

AB - We have detected the dynamics of 17-mer DNA triplex dissociation mechanism at the molecular level. Fluorescence resonance energy transfer (FRET) was used as an indicator of intermolecular interaction in nanometer range, whereas atomic force microscopy (AFM) was employed to address single molecule with sub-angstrom precision. The maximum rupture force of DNA triplex was found at pH 4.65, consistent with macroscopic observations. These results indicated that the FRET together with an AFM detection system could be used to reveal the DNA triplex interaction in nanometer scale unambiguously.

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U2 - 10.1063/1.2809406

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VL - 91

JO - Applied Physics Letters

JF - Applied Physics Letters

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ER -

Direct visualization of triplex DNA molecular dynamics by fluorescence resonance energy transfer and atomic force microscopy measurements

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