Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films

Fabio Palumbo*, Giuseppe Camporeale, Yi Wei Yang, Jong-Shinn Wu, Eloisa Sardella, Giorgio Dilecce, Cosima Damiana Calvano, Laura Quintieri, Leonardo Caputo, Federico Baruzzi, Pietro Favia

*此作品的通信作者

研究成果: Article同行評審

33 引文 斯高帕斯(Scopus)

摘要

Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14 μg/cm2 as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test.

原文English
頁(從 - 到)1302-1310
頁數9
期刊Plasma Processes and Polymers
12
發行號11
DOIs
出版狀態Published - 1 十一月 2015

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