Differentiation between normal and cancer cells with autofluorescence lifetime microscopy and nanosecond pulsed electric field effects

Nobuhiro Ohta, Kamlesh Awasthi, Hsin-Yun Hsu, Pei Ying Hsieh

研究成果: Conference contribution同行評審

1 引文 斯高帕斯(Scopus)

摘要

Fluorescence lifetime images of the endogenous nicotinamide adenine dinucleotide (NADH), which is well known as autofluorescence chromophores, were obtained for rat normal fibroblast cells (WFB) and H-ras oncogene-transfected WFB cancer cells (W31) and for human normal lung fibroblast (MRC-5) and human lung large carcinoma (HCI-H661). In both cases, the average lifetime of the NADH autofluorescence was shorter in cancer cells than in normal cells, indicating that the difference in metabolism between healthy and cancer cells might alter the level of coenzymes such as NADH. It is also shown that application of nanosecond pulsed electric field (nsPEF) induces apoptosis in these cell, resulting in the morphological changes and lengthening the autofluorescence lifetime of NADH. Furthermore, we found that nsPEF more efficiently affected cancer cells than normal cells in cell viability, suggesting the possibility of drug-free cancer therapy by nsPEF.

原文English
主出版物標題Label-Free Biomedical Imaging and Sensing, LBIS 2019
編輯Natan T. Shaked, Oliver Hayden
發行者SPIE
ISBN(電子)9781510624221
DOIs
出版狀態Published - 4 三月 2019
事件Label-Free Biomedical Imaging and Sensing 2019, LBIS 2019 - San Francisco, United States
持續時間: 2 二月 20195 二月 2019

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
10890
ISSN(列印)1605-7422

Conference

ConferenceLabel-Free Biomedical Imaging and Sensing 2019, LBIS 2019
國家/地區United States
城市San Francisco
期間2/02/195/02/19

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