TY - GEN
T1 - Differentiation between normal and cancer cells with autofluorescence lifetime microscopy and nanosecond pulsed electric field effects
AU - Ohta, Nobuhiro
AU - Awasthi, Kamlesh
AU - Hsu, Hsin-Yun
AU - Hsieh, Pei Ying
PY - 2019/3/4
Y1 - 2019/3/4
N2 - Fluorescence lifetime images of the endogenous nicotinamide adenine dinucleotide (NADH), which is well known as autofluorescence chromophores, were obtained for rat normal fibroblast cells (WFB) and H-ras oncogene-transfected WFB cancer cells (W31) and for human normal lung fibroblast (MRC-5) and human lung large carcinoma (HCI-H661). In both cases, the average lifetime of the NADH autofluorescence was shorter in cancer cells than in normal cells, indicating that the difference in metabolism between healthy and cancer cells might alter the level of coenzymes such as NADH. It is also shown that application of nanosecond pulsed electric field (nsPEF) induces apoptosis in these cell, resulting in the morphological changes and lengthening the autofluorescence lifetime of NADH. Furthermore, we found that nsPEF more efficiently affected cancer cells than normal cells in cell viability, suggesting the possibility of drug-free cancer therapy by nsPEF.
AB - Fluorescence lifetime images of the endogenous nicotinamide adenine dinucleotide (NADH), which is well known as autofluorescence chromophores, were obtained for rat normal fibroblast cells (WFB) and H-ras oncogene-transfected WFB cancer cells (W31) and for human normal lung fibroblast (MRC-5) and human lung large carcinoma (HCI-H661). In both cases, the average lifetime of the NADH autofluorescence was shorter in cancer cells than in normal cells, indicating that the difference in metabolism between healthy and cancer cells might alter the level of coenzymes such as NADH. It is also shown that application of nanosecond pulsed electric field (nsPEF) induces apoptosis in these cell, resulting in the morphological changes and lengthening the autofluorescence lifetime of NADH. Furthermore, we found that nsPEF more efficiently affected cancer cells than normal cells in cell viability, suggesting the possibility of drug-free cancer therapy by nsPEF.
KW - Cancer and normal cells
KW - Endogenous fluorophore
KW - Fluorescence lifetime microscopy (FLIM)
KW - Nanosecond pulsed electric field effect
KW - Nicotinamide adenine dinucleotide
UR - http://www.scopus.com/inward/record.url?scp=85064874329&partnerID=8YFLogxK
U2 - 10.1117/12.2509164
DO - 10.1117/12.2509164
M3 - Conference contribution
AN - SCOPUS:85064874329
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Label-Free Biomedical Imaging and Sensing, LBIS 2019
A2 - Shaked, Natan T.
A2 - Hayden, Oliver
PB - SPIE
T2 - Label-Free Biomedical Imaging and Sensing 2019, LBIS 2019
Y2 - 2 February 2019 through 5 February 2019
ER -