Differential Releases of Dopamine and Neuropeptide Y from Histamine-Stimulated PC12 Cells Detected by an Aptamer-Modified Nanowire Transistor

Subhasree  Banerjee; Ying Jhu Hsieh; Chia Rung Liu; Nai-Hsing  Yeh; Hui-Hsing  Hung; Yew-Seng Lai; Ai-Chuan Chou; Yit-Tsong Chen; Chien Yuan Pan

Differential Releases of Dopamine and Neuropeptide Y from Histamine-Stimulated PC12 Cells Detected by an Aptamer-Modified Nanowire Transistor

Subhasree Banerjee, Ying Jhu Hsieh, Chia Rung Liu, Nai-Hsing Yeh, Hui-Hsing Hung, Yew-Seng Lai, Ai-Chuan Chou, Yit-Tsong Chen^*, Chien Yuan Pan^*

^*此作品的通信作者

力積電-陽明交大研究中心

研究成果: Article › 同行評審

20 引文斯高帕斯（Scopus）

摘要

Dopamine (DA) and neuropeptide Y (NPY) are important neurotransmitters stored mainly in synaptic vesicles (SV) and large dense core vesicles (LDCV), respectively. SV and LDCV may respond differentially to various stimulations, resulting in the release of the neurotransmitters in different ratios to modulate physiological activities. However, it is difficult to monitor these neurotransmitters in real-time, especially NPY, which is usually detected by time-consuming
techniques, such as the Western blot. In this study, we detected the histamine-induced NPY and DA released from pheochromocytoma 12 (PC12) cells using silicon nanowire field-effect transistors (SiNW-FETs) modified with specific
aptamers against NPY and DA, respectively (referred to as
APTNPY/SiNW-FET and APTDA/SiNW-FET).

原文	American English
頁數	5524
期刊	Communications
卷	12
發行號	40
出版狀態	Published - 2016

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2016 Differential Releases of Dopamine and Neuropeptide Y from Histamine-Stimulated PC12 Cells Detected by an Aptamer-Modifi ed Nanowire Transistor

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title = "Differential Releases of Dopamine and Neuropeptide Y from Histamine-Stimulated PC12 Cells Detected by an Aptamer-Modified Nanowire Transistor",

abstract = "Dopamine (DA) and neuropeptide Y (NPY) are important neurotransmitters stored mainly in synaptic vesicles (SV) and large dense core vesicles (LDCV), respectively. SV and LDCV may respond differentially to various stimulations, resulting in the release of the neurotransmitters in different ratios to modulate physiological activities. However, it is difficult to monitor these neurotransmitters in real-time, especially NPY, which is usually detected by time-consumingtechniques, such as the Western blot. In this study, we detected the histamine-induced NPY and DA released from pheochromocytoma 12 (PC12) cells using silicon nanowire field-effect transistors (SiNW-FETs) modified with specificaptamers against NPY and DA, respectively (referred to asAPTNPY/SiNW-FET and APTDA/SiNW-FET). ",

author = "Subhasree Banerjee and Hsieh, {Ying Jhu} and Liu, {Chia Rung} and Nai-Hsing Yeh and Hui-Hsing Hung and Yew-Seng Lai and Ai-Chuan Chou and Yit-Tsong Chen and Pan, {Chien Yuan}",

year = "2016",

language = "American English",

volume = "12",

journal = "Communications",

issn = "0341-2059",

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T1 - Differential Releases of Dopamine and Neuropeptide Y from Histamine-Stimulated PC12 Cells Detected by an Aptamer-Modified Nanowire Transistor

AU - Banerjee, Subhasree

AU - Hsieh, Ying Jhu

AU - Liu, Chia Rung

AU - Yeh, Nai-Hsing

AU - Hung, Hui-Hsing

AU - Lai, Yew-Seng

AU - Chou, Ai-Chuan

AU - Chen, Yit-Tsong

AU - Pan, Chien Yuan

PY - 2016

Y1 - 2016

N2 - Dopamine (DA) and neuropeptide Y (NPY) are important neurotransmitters stored mainly in synaptic vesicles (SV) and large dense core vesicles (LDCV), respectively. SV and LDCV may respond differentially to various stimulations, resulting in the release of the neurotransmitters in different ratios to modulate physiological activities. However, it is difficult to monitor these neurotransmitters in real-time, especially NPY, which is usually detected by time-consumingtechniques, such as the Western blot. In this study, we detected the histamine-induced NPY and DA released from pheochromocytoma 12 (PC12) cells using silicon nanowire field-effect transistors (SiNW-FETs) modified with specificaptamers against NPY and DA, respectively (referred to asAPTNPY/SiNW-FET and APTDA/SiNW-FET).

AB - Dopamine (DA) and neuropeptide Y (NPY) are important neurotransmitters stored mainly in synaptic vesicles (SV) and large dense core vesicles (LDCV), respectively. SV and LDCV may respond differentially to various stimulations, resulting in the release of the neurotransmitters in different ratios to modulate physiological activities. However, it is difficult to monitor these neurotransmitters in real-time, especially NPY, which is usually detected by time-consumingtechniques, such as the Western blot. In this study, we detected the histamine-induced NPY and DA released from pheochromocytoma 12 (PC12) cells using silicon nanowire field-effect transistors (SiNW-FETs) modified with specificaptamers against NPY and DA, respectively (referred to asAPTNPY/SiNW-FET and APTDA/SiNW-FET).

M3 - Article

SN - 0341-2059

VL - 12

JO - Communications

JF - Communications

IS - 40

ER -

Differential Releases of Dopamine and Neuropeptide Y from Histamine-Stimulated PC12 Cells Detected by an Aptamer-Modified Nanowire Transistor

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