Differential regulation of prostaglandin endoperoxide synthase-2 transcription in ovine granulosa and large luteal cells

Yuh Lin Wu, Milo C. Wiltbank*

*此作品的通信作者

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11 引文 斯高帕斯(Scopus)

摘要

The objective of the present study was to evaluate second messenger regulation of prostaglandin synthase-2 (PGS-2) mRNA and PGS-2 promoter in ovine granulosa cells and large luteal cells. In granulosa cells, PGS-2 mRNA was induced by forskolin (PKA activator) but not by phorbol didecanoate (PDD; PKC activator) with maximal stimulation at 24 h. In contrast, PDD was the more potent inducer in large luteal cells with the most dramatic effect by 4 h. Similarly, forskolin but not PDD increased media PGF in granulosa cells at 24 h; whereas, PDD but not forskolin increased PGF at 4 h and 24 h in large luteal cells. To evaluate if these effects were due to activation of transcription, a plasmid was constructed containing 1500 bp of PGS-2 promoter linked to a luciferase gene. Forskolin stimulated transcription from this construct in granulosa cells (5-fold); whereas, PDD but not forskolin stimulated transcription in large luteal cells (40-fold). Taking these findings together, we propose that transcriptional regulation of the PGS-2 gene changes from PKA-dependence in granulosa cells to PKC-dependence in large luteal cells after luteinization.

原文English
頁(從 - 到)103-116
頁數14
期刊Prostaglandins and Other Lipid Mediators
65
發行號2-3
DOIs
出版狀態Published - 2001

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