Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica

Shih Yu Liang; Yun Hsien Chan; Kan Tai Hsia; Jing Lun Lee; Ming Chu Kuo; Kuo Yuan Hwa; Chi Wen Chan; Ting Yi Chiang; Jung Sheng Chen; Fang Tzy Wu; Dar Der Ji

doi:10.1128/JCM.00105-09

Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica

Shih Yu Liang, Yun Hsien Chan, Kan Tai Hsia, Jing Lun Lee, Ming Chu Kuo, Kuo Yuan Hwa, Chi Wen Chan, Ting Yi Chiang, Jung Sheng Chen, Fang Tzy Wu, Dar Der Ji^*

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研究成果: Article › 同行評審

67 引文斯高帕斯（Scopus）

摘要

A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.

原文	English
頁（從 - 到）	1892-1895
頁數	4
期刊	Journal of Clinical Microbiology
卷	47
發行號	6
DOIs	https://doi.org/10.1128/JCM.00105-09
出版狀態	Published - 6月 2009

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abstract = "A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.",

author = "Liang, {Shih Yu} and Chan, {Yun Hsien} and Hsia, {Kan Tai} and Lee, {Jing Lun} and Kuo, {Ming Chu} and Hwa, {Kuo Yuan} and Chan, {Chi Wen} and Chiang, {Ting Yi} and Chen, {Jung Sheng} and Wu, {Fang Tzy} and Ji, {Dar Der}",

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AU - Hsia, Kan Tai

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AU - Kuo, Ming Chu

AU - Hwa, Kuo Yuan

AU - Chan, Chi Wen

AU - Chiang, Ting Yi

AU - Chen, Jung Sheng

AU - Wu, Fang Tzy

AU - Ji, Dar Der

PY - 2009/6

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Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica

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