Determining early adhesion of cells on polysaccharides/PCL surfaces by a quartz crystal microbalance

Tze Wen Chung*, Yu Chang Tyan, Rong Ho Lee, Chia Wei Ho

*此作品的通信作者

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

The early adhesions of cells to various biopolymers are important to their growths and proliferations. Here, the adhesion of cells (e.g., fibroblasts) on the electrode of a quartz crystal microbalance (QCM) that was coated by PCL or PEG/ PCL and further adsorbed by chitosan (CS) or CS/hyaluronic acid (HA) layers, was examined by cell-counting technique, QCMmethod and MTS assay under a serum-free condition for 3 h. The surfaces on electrodes of the QCM were confirmed to have been modified by measuring their contact angles, FT-IR spectra and the weights of biopolymers affected the frequency shifts of the QCM. Among tested surfaces on electrodes, the adhesion of fibroblasts on a HA/CS/PCL surface was the most (e.g., 3.08 × 105 cells/cm2) while that on a PEG/PCL surface was the least (e.g., 0.7 × 105 cells/cm2), as determined by cell-counting technique. The frequency shift and the mass of adhering fibroblasts on HA/CS/PCL electrodes were -3,537 ± 770 Hz and 3.78 ± 0.22 μg (n = 3), respectively, that were significantly exceeded those on other electrodes (-393 ± 58 Hz and 0.32 ± 0.06 μg, n = 3, respectively, for PEG/PCL electrodes). These results were consistent with cellcounting technique. Although MTS assay yielded similar results, it was less sensitive than the two aforementioned methods. In conclusion, modified electrodes of aQCMprovide a convenient and sensitive method for examining the early adhesion of cells (e.g., 3 h) to biopolymer surfaces.

原文English
頁(從 - 到)3067-3073
頁數7
期刊Journal of Materials Science: Materials in Medicine
23
發行號12
DOIs
出版狀態Published - 12月 2012

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