Determination of three-dimensional molecular orientation of type-I collagen by circularly-polarized second harmonic generation imaging

Guan Yu Zhuo, Wei Han Hung, Fu Jen Kao

研究成果: Conference contribution同行評審

1 引文 斯高帕斯(Scopus)

摘要

The content of collagen is up to 30% existing in mammals. It supports the main component of connective tissues such as skin, ligament, and cartilage. Among various types of collagen, type-I collagen is of the most abundance and has been broadly studied due to the importance in bioscience. Second harmonic generation (SHG) microscopy is an effective tool used to study the collagen organization without labeling. In this study, we used circular polarization instead of linear polarization to retrieve three-dimensional (3D) molecular orientation of type-I collagen with only two cross polarized SHG images without acquiring an image stack of varying polarization.

原文English
主出版物標題Biomedical Imaging and Sensing Conference
編輯Yasuhiro Awatsuji, Osamu Matoba, Yoshihisa Aizu, Toyohiko Yatagai
發行者SPIE
ISBN(電子)9781510610033
DOIs
出版狀態Published - 2017
事件3rd Biomedical Imaging and Sensing Conference - Yokohama, 日本
持續時間: 19 4月 201721 4月 2017

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
10251
ISSN(列印)0277-786X
ISSN(電子)1996-756X

Conference

Conference3rd Biomedical Imaging and Sensing Conference
國家/地區日本
城市Yokohama
期間19/04/1721/04/17

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