Detection of tetracycline using isothermal amplification

Chia Cheng Wu, Li An Wu, Yih Fan Chen, Lu Ting Chou

研究成果: Conference contribution同行評審

1 引文 斯高帕斯(Scopus)

摘要

PCR is an important biosensing method since it can amplify DNA by many orders of magnitude. However, for food safety and environmental monitoring, the target molecule is usually not DNA. Here we demonstrate a detection method that integrates competitive assay format, magnetic bead separation, and isothermal DNA amplification for sensitive detection of tetracycline (TET) and other molecules. While TET is an antibiotic rather than oligonucleotide, our method allows the signal of TET detection to be amplified like conventional PCR. In addition, since we use isothermal amplification instead of conventional PCR, the temperature of the sample solution only needs to be maintained at a fixed temperature during the amplification process. We have successfully used the method to detect 10 pM TET. In addition, to improve the sensitivity of detection further, besides using EvaGreen dyes to quantify the amplified DNA, we have also tested another DNA quantification method that uses graphene oxide and a fluorescent DNA probe to increase the signal to background ratio of fluorescence detection.

原文English
主出版物標題2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
發行者Institute of Electrical and Electronics Engineers Inc.
頁面210-213
頁數4
ISBN(電子)9781509030590
DOIs
出版狀態Published - 25 8月 2017
事件12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States
持續時間: 9 4月 201712 4月 2017

出版系列

名字2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Conference

Conference12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
國家/地區United States
城市Los Angeles
期間9/04/1712/04/17

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