Design of PCR primers for the mcrA genes detection of methanogen communities

Nguyen Ngoc Tuan, Shir Ly Huang*

*此作品的通信作者

研究成果: Conference contribution同行評審

2 引文 斯高帕斯(Scopus)

摘要

Methanogens play an important role to carbon cycling, catalyzing the production of methane and carbon dioxide, both potent green house gases, during organic matter degradation in anaerobic environments. Therefore, it is necessary to better understand microorganisms that produce natural gas. Indeed, methanogens are difficult to perform through culture based methods. In addition, the culture independent methods using the 16S rRNA gene also revealed some disadvantages. For these reasons, the culture independent molecular techniques using the specific catabolic genes such as methyl coenzyme M reductase (MCR) were studied. In this study, a primer set which can amplify specific fragments from a wide variety of mcrA gene was designed based on the homologous regions of 100 mcrA genes listed in the GenBank. PCR with the mcrA primers amplified DNA fragments of the expected size from all the six samples which obtained from biogas production reactors. In addition, denaturing gradient gel electrophoresis PCR analysis using our designed primers also revealed the diversity of mcrA gene in each sample. These results revealed that our primers were successfully to detect the mcrA genes and it is also helpful to know the diversity of mcrA genes in methanogen communities.

原文English
主出版物標題Advances in Science and Engineering II
頁面408-413
頁數6
DOIs
出版狀態Published - 2012
事件2011 WASE Global Conference on Science Engineering, GCSE 2011 - Taiyuan and Xian, China
持續時間: 10 12月 201111 12月 2011

出版系列

名字Applied Mechanics and Materials
135-136
ISSN(列印)1660-9336
ISSN(電子)1662-7482

Conference

Conference2011 WASE Global Conference on Science Engineering, GCSE 2011
國家/地區China
城市Taiyuan and Xian
期間10/12/1111/12/11

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