TY - JOUR
T1 - Description of the transcriptomes of immune response-activated hemocytes from the mosquito vectors Aedes aegypti and Armigeres subalbatus
AU - Bartholomay, Lyric C.
AU - Cho, Wen Long
AU - Rocheleau, Thomas A.
AU - Boyle, Jon P.
AU - Beck, Eric T.
AU - Fuchs, Jeremy F.
AU - Liss, Paul
AU - Rusch, Michael
AU - Butler, Katherine M.
AU - Wu, Roy Chen Chih
AU - Lin, Shih Pei
AU - Kuo, Hang Yen
AU - Tsao, I. Yu
AU - Huang, Chiung Yin
AU - Liu, Tze Tze
AU - Hsiao, Kwang Jen
AU - Tsai, Shih Feng
AU - Yang, Ueng Cheng
AU - Nappi, Anthony J.
AU - Perna, Nicole T.
AU - Chen, Chen Cheng
AU - Christensen, Bruce M.
PY - 2004/7
Y1 - 2004/7
N2 - Mosquito-borne diseases, including dengue, malaria, and lymphatic filariasis, exact a devastating toll on global health and economics, killing or debilitating millions every year (54). Mosquito innate immune responses are at the forefront of concerted research efforts aimed at defining potential target genes that could be manipulated to engineer pathogen resistance in vector populations. We aimed to describe the pivotal role that circulating blood cells (called hemocytes) play in immunity by generating a total of 11,952 Aedes aegypti and 12,790 Armigeres subalbatus expressed sequence tag (EST) sequences from immune response-activated hemocyte libraries. These ESTs collapsed into 2,686 and 2,107 EST clusters, respectively. The clusters were used to adapt the web-based interface for annotating bacterial genomes called A Systematic Annotation Package for Community Analysis of Genomes (ASAP) for analysis of ESTs. Each cluster was categorically characterized and annotated in ASAP based on sequence similarity to five sequence databases. The sequence data and annotations can be viewed in ASAP at https://asap.ahabs.wisc.edu/annotation/php/ ASAP1.htm. The data presented here represent the results of the first high-throughput in vivo analysis of the transcriptome of immunocytes from an invertebrate. Among the sequences are those for numerous immunity-related genes, many of which parallel those employed in vertebrate innate immunity, that have never been described for these mosquitoes.
AB - Mosquito-borne diseases, including dengue, malaria, and lymphatic filariasis, exact a devastating toll on global health and economics, killing or debilitating millions every year (54). Mosquito innate immune responses are at the forefront of concerted research efforts aimed at defining potential target genes that could be manipulated to engineer pathogen resistance in vector populations. We aimed to describe the pivotal role that circulating blood cells (called hemocytes) play in immunity by generating a total of 11,952 Aedes aegypti and 12,790 Armigeres subalbatus expressed sequence tag (EST) sequences from immune response-activated hemocyte libraries. These ESTs collapsed into 2,686 and 2,107 EST clusters, respectively. The clusters were used to adapt the web-based interface for annotating bacterial genomes called A Systematic Annotation Package for Community Analysis of Genomes (ASAP) for analysis of ESTs. Each cluster was categorically characterized and annotated in ASAP based on sequence similarity to five sequence databases. The sequence data and annotations can be viewed in ASAP at https://asap.ahabs.wisc.edu/annotation/php/ ASAP1.htm. The data presented here represent the results of the first high-throughput in vivo analysis of the transcriptome of immunocytes from an invertebrate. Among the sequences are those for numerous immunity-related genes, many of which parallel those employed in vertebrate innate immunity, that have never been described for these mosquitoes.
UR - http://www.scopus.com/inward/record.url?scp=3042641031&partnerID=8YFLogxK
U2 - 10.1128/IAI.72.7.4114-4126.2004
DO - 10.1128/IAI.72.7.4114-4126.2004
M3 - Article
C2 - 15213157
AN - SCOPUS:3042641031
SN - 0019-9567
VL - 72
SP - 4114
EP - 4126
JO - Infection and Immunity
JF - Infection and Immunity
IS - 7
ER -
