DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

Sheng Fan Wang; Jason C. Huang; Yuan Ming Lee; Shih Jen Liu; Yu Jiun Chan; Yat Pang Chau; Pele Chong; Yi Ming Arthur Chen

doi:10.1016/j.bbrc.2008.06.078

DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

Sheng Fan Wang, Jason C. Huang, Yuan Ming Lee, Shih Jen Liu, Yu Jiun Chan, Yat Pang Chau, Pele Chong, Yi Ming Arthur Chen^*

^*此作品的通信作者

醫學生物技術暨檢驗學系

研究成果: Article › 同行評審

76 引文斯高帕斯（Scopus）

摘要

DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.

原文	English
頁（從 - 到）	561-566
頁數	6
期刊	Biochemical and Biophysical Research Communications
卷	373
發行號	4
DOIs	https://doi.org/10.1016/j.bbrc.2008.06.078
出版狀態	Published - 5 9月 2008

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10.1016/j.bbrc.2008.06.078

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title = "DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans",

abstract = "DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.",

keywords = "DC-SIGN, Dendritic cells, H5N1, Pseudotyped virus",

author = "Wang, {Sheng Fan} and Huang, {Jason C.} and Lee, {Yuan Ming} and Liu, {Shih Jen} and Chan, {Yu Jiun} and Chau, {Yat Pang} and Pele Chong and Chen, {Yi Ming Arthur}",

note = "Funding Information: Our gratitude goes to the Vaccine Research and Development Center, National Health Research Institute, Taiwan for providing H5N1 A/Vietnam1194/04 RG strain and to members of Dr. Shie-Liang Hsieh{\textquoteright}s lab for their advice and support for human DC isolation. This work was supported by a grant from the National Science Council, Republic of China (NSC 96-2321-B-010-007) and Aim for the Top University Plan. ",

year = "2008",

month = sep,

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doi = "10.1016/j.bbrc.2008.06.078",

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T1 - DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

AU - Wang, Sheng Fan

AU - Huang, Jason C.

AU - Lee, Yuan Ming

AU - Liu, Shih Jen

AU - Chan, Yu Jiun

AU - Chau, Yat Pang

AU - Chong, Pele

AU - Chen, Yi Ming Arthur

N1 - Funding Information: Our gratitude goes to the Vaccine Research and Development Center, National Health Research Institute, Taiwan for providing H5N1 A/Vietnam1194/04 RG strain and to members of Dr. Shie-Liang Hsieh’s lab for their advice and support for human DC isolation. This work was supported by a grant from the National Science Council, Republic of China (NSC 96-2321-B-010-007) and Aim for the Top University Plan.

PY - 2008/9/5

Y1 - 2008/9/5

N2 - DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.

AB - DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.

KW - DC-SIGN

KW - Dendritic cells

KW - H5N1

KW - Pseudotyped virus

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JO - Biochemical and Biophysical Research Communications

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ER -

DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

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