Conversion of human umbilical cord mesenchymal stem cells in Wharton's Jelly to dopaminergic neurons in vitro: Potential therapeutic application for Parkinsonism

Yu Show Fu*, Yun Chih Cheng, Maan Yuh Anya Lin, Henrich Cheng, Pei Ming Chu, Shih Chich Chou, Yang Hsin Shih, Miau Hwa Ko, Min Shan Sung

*此作品的通信作者

研究成果: Article同行評審

381 引文 斯高帕斯(Scopus)

摘要

Human mesenchymal stem cells isolated from Wharton's jelly of the umbilical cord were induced to transform into dopaminergic neurons in vitro through stepwise culturing in neuron-conditioned medium, sonic hedgehog, and FGF8. The success rate was 12.7%, as characterized by positive staining for tyrosine hydroxylase (TH), the rate-limiting catecholaminergic synthesizing enzyme, and dopamine being released into the culture medium. Transplantation of such cells into the striatum of rats previously made Parkinsonian by unilateral striatal lesioning with the dopaminergic neurotoxin 6-hydroxydopamine partially corrected the lesion-induced amphetamine-evoked rotation. Viability of the transplanted cells at least 4 months after transplantation was identified by positive TH staining and migration of 1.4 mm both rostrally and caudally. These results suggest that human umbilical mesenchymal stem cells have the potential for treatment of Parkinson's disease.

原文English
頁(從 - 到)115-124
頁數10
期刊Stem Cells
24
發行號1
DOIs
出版狀態Published - 1月 2006

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