Comparison of backward-scattered detection and forward-scattered detection for measuring optical force in optical tweezers

Yi Jr Su*, Long Hsu

*此作品的通信作者

研究成果: Conference contribution同行評審

摘要

Optical tweezers have become an important tool to measure forces in biology. The trapped particle displacements acquired from the position detection system are applied to calibrate trapping stiffness using power spectrum method. The near infrared light is typically used as a laser source to reduce the damage to a cell or cellular organelles and the biological objects can be held and moved by exerting piconewton forces. In force measurement, optical force strength is calculated by multiplying trapping stiffness and trapped bead displacement. Optical tweezers perform a wider range of experiments through the integration of a quadrant photodiode for position detection. Both forward-scattered detection and backward-scattered detection are the typical position detection. This study discussed both backward-scattered detection and forward-scattered detection that add a probing beam and their linear detection ranges that describe the precise position of the trapped bead. This work also discussed their linear detection ranges related to the distance between the two laser system focuses, confirming the optimum positions of the two focuses. The result indicated that the linear detection range of backward-scattered detection is longer than the forward-scattered detection. Hence, backwardscattered detection measures the longer displacement of the trapped bead in optical force measurement.

原文English
主出版物標題Optical Trapping and Optical Micromanipulation VII
DOIs
出版狀態Published - 27 10月 2010
事件Optical Trapping and Optical Micromanipulation VII - San Diego, CA, United States
持續時間: 1 8月 20105 8月 2010

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
7762
ISSN(列印)0277-786X

Conference

ConferenceOptical Trapping and Optical Micromanipulation VII
國家/地區United States
城市San Diego, CA
期間1/08/105/08/10

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