The coherent injection-locking and directly modulation of a long-cavity colorless laser diode with 1% end-facet reflectance and weak-resonant longitudinal modes is employed as an universal optical transmitter to demonstrated for optical 16-QAM OFDM transmission at 12 Gbit/s over 25 km in a DWDM-PON system. The optimized bias current of 30 mA (∼1.5Ith) with corresponding extinction ratio (ER) of 6 dB and the external injection power of 9 dBm is (are) required for such a wavelength-locked universal transmitter to carry the 16-QAM and 122-subcarrier formatted OFDM and data-stream. By increasing external injection-locking from 9 dBm to 0 dBm, the peak-to-peak chirp of the OFDM data stream reduces from 7.7 to 5.4 GHz. The side mode suppression ratio (SMSR) of up to 50 dB is achieved with wider detuning range between 5 nm to 2.0 nm under an injection power of 0 dBm. By modulating such a colorless laser diode with an OFDM data stream of 122 subcarriers at a central carrier frequency of 1.5625 GHz and a total bandwidth of 3 GHz, the transmission data rate of up to 12 Gbit/s in standard single-mode fiber over 25 km is demonstrated to achieve an error vector magnitude (EVM) of 5.435%. Such a universal colorless DWDM-PON transmitter can deliver the optical OFDM data-stream at 12 Gbit/s QAM-OFDM data after 25-km transmission with a receiving power sensitivity of 7 dBm at BER of 3.6× 107 when pre-amplifying the OFDM data by 5 dB.