Characterization of restriction endonuclease maps of hepatitis B viral DNAS

Szecheng J. Lo; Yan-Hwa Wu Lee; Jang Shing Chiou; Ling Pai Ting; Wu Tse Liu; Kong Bung Choo

doi:10.1016/0006-291X(85)91962-X

Characterization of restriction endonuclease maps of hepatitis B viral DNAS

Szecheng J. Lo^*, Yan-Hwa Wu Lee, Jang Shing Chiou, Ling Pai Ting, Wu Tse Liu, Kong Bung Choo

^*此作品的通信作者

生物科技學系

研究成果: Article › 同行評審

25 引文斯高帕斯（Scopus）

摘要

The HBV DNA isolated from Dane particles of 9 patients plasm was cloned into the EcoRI or BamHI site of the pUC8 plasmids containing plasmids with full length HBV DNA and four plasmids containing the HBV surface antigen gene were obtained. Based on our cloned HBV DNA and a comparison with 7 complete sequences and 5 restriction endonuclease patterns of HBV DNA published by others, we can recognize common restriction sites shared by different subtypes (adw, adr, ayw, and adyw): (1) a HincII site in the S gene, (2) a BamHI site in the X region, and (3) two BglII sites in the C gene. In addition adw has specific sites for HincII, BamHI, and PstI in the pre-S region. A unique XhoI site is present in the pre-S region in all subtypes except for adw.

原文	English
頁（從 - 到）	797-803
頁數	7
期刊	Biochemical and Biophysical Research Communications
卷	129
發行號	3
DOIs	https://doi.org/10.1016/0006-291X(85)91962-X
出版狀態	Published - 28 6月 1985

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title = "Characterization of restriction endonuclease maps of hepatitis B viral DNAS",

abstract = "The HBV DNA isolated from Dane particles of 9 patients plasm was cloned into the EcoRI or BamHI site of the pUC8 plasmids containing plasmids with full length HBV DNA and four plasmids containing the HBV surface antigen gene were obtained. Based on our cloned HBV DNA and a comparison with 7 complete sequences and 5 restriction endonuclease patterns of HBV DNA published by others, we can recognize common restriction sites shared by different subtypes (adw, adr, ayw, and adyw): (1) a HincII site in the S gene, (2) a BamHI site in the X region, and (3) two BglII sites in the C gene. In addition adw has specific sites for HincII, BamHI, and PstI in the pre-S region. A unique XhoI site is present in the pre-S region in all subtypes except for adw.",

author = "Lo, {Szecheng J.} and {Wu Lee}, Yan-Hwa and Chiou, {Jang Shing} and Ting, {Ling Pai} and Liu, {Wu Tse} and Choo, {Kong Bung}",

year = "1985",

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AU - Lo, Szecheng J.

AU - Wu Lee, Yan-Hwa

AU - Chiou, Jang Shing

AU - Ting, Ling Pai

AU - Liu, Wu Tse

AU - Choo, Kong Bung

PY - 1985/6/28

Y1 - 1985/6/28

N2 - The HBV DNA isolated from Dane particles of 9 patients plasm was cloned into the EcoRI or BamHI site of the pUC8 plasmids containing plasmids with full length HBV DNA and four plasmids containing the HBV surface antigen gene were obtained. Based on our cloned HBV DNA and a comparison with 7 complete sequences and 5 restriction endonuclease patterns of HBV DNA published by others, we can recognize common restriction sites shared by different subtypes (adw, adr, ayw, and adyw): (1) a HincII site in the S gene, (2) a BamHI site in the X region, and (3) two BglII sites in the C gene. In addition adw has specific sites for HincII, BamHI, and PstI in the pre-S region. A unique XhoI site is present in the pre-S region in all subtypes except for adw.

AB - The HBV DNA isolated from Dane particles of 9 patients plasm was cloned into the EcoRI or BamHI site of the pUC8 plasmids containing plasmids with full length HBV DNA and four plasmids containing the HBV surface antigen gene were obtained. Based on our cloned HBV DNA and a comparison with 7 complete sequences and 5 restriction endonuclease patterns of HBV DNA published by others, we can recognize common restriction sites shared by different subtypes (adw, adr, ayw, and adyw): (1) a HincII site in the S gene, (2) a BamHI site in the X region, and (3) two BglII sites in the C gene. In addition adw has specific sites for HincII, BamHI, and PstI in the pre-S region. A unique XhoI site is present in the pre-S region in all subtypes except for adw.

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Characterization of restriction endonuclease maps of hepatitis B viral DNAS

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