Ca2+-binding protein centrin 4 is a novel binding partner of rat Eag1 K+ channels

Po Hao Hsu, Yi Chih Chiu, Ting Feng Lin, Chung Jiuan Jeng*

*此作品的通信作者

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

Eag1 is neuron-specific K+ channel abundantly expressed in the brain and retina. Subcellular localization and physiological analyses in neurons reveal that Eag1 may participate in Ca2+-signaling processes in the synapse. Here, we searched for rat Eag1 (rEag1)-binding proteins that may contribute to Ca2+ regulation of the K+ channel. Yeast two-hybrid screening identified centrin 4, a member of the centrin family of Ca2+-binding proteins. GST pull-down and immunoprecipitation assays in brain and retina lysates confirm the interaction of centrin with rEag1 in neurons. Centrin 4 binds to rEag1 in the absence of Ca2+. Raising Ca2+ concentration enhances the association efficiency of centrin 4 and rEag1, and is required for the suppression of rEag1 currents by centrin 4. Altogether, our data suggest that centrin 4 is a novel binding partner that may contribute to Ca2+ regulation of rEag1 in neurons. Centrin 4 is a member of the centrin family of Ca2+-binding proteins and has been identified as binding to the K+ channel rEag1 in neurons. Unlike camodulin, centrin 4 binds to rEag1 in the absence of Ca2+. However, raising Ca2+ concentration enhances the association efficiency of centrin 4 and rEag1, and is required for the suppression of rEag1 currents by centrin 4.

原文English
頁(從 - 到)349-357
頁數9
期刊FEBS Open Bio
6
發行號4
DOIs
出版狀態Published - 1 4月 2016

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