TY - JOUR
T1 - Bright carbon dots as fluorescence sensing agents for bacteria and curcumin
AU - Baig, Mirza Muhammad Fahad
AU - Chen, Yu-Chie
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Carbon dots (C-dots) are fluorescent nanomaterials that possess good photostability and low toxicity. They have been used as sensing probes and bioimaging agents for a variety of biological species. Numerous methods are available to generate C-dots. Nevertheless, simple and straightforward synthesis methods must be explored for the synthesis of C-dots from inexpensive, natural sources. In this study, we developed a simple method to generate C-dots from inexpensive chicken egg whites through a one-step heating reaction. The size of the generated C-dots was 3.3 ± 0.4 nm, and the quantum yield of the C-dots was as high as ∼43%. The as-prepared C-dots can be used as multicolor labeling agents for bacteria such as Staphylococcus aureus and Escherichia coli. Furthermore, the generated C-dots can be used as Förster resonance energy transfer sensing probes for curcumin, which is an active ingredient of turmeric and medicinal pigment. The feasibility of using the C-dots as selective sensing probes to determine the amount of curcumin from complex turmeric powder and condensed turmeric tablets is also demonstrated.
AB - Carbon dots (C-dots) are fluorescent nanomaterials that possess good photostability and low toxicity. They have been used as sensing probes and bioimaging agents for a variety of biological species. Numerous methods are available to generate C-dots. Nevertheless, simple and straightforward synthesis methods must be explored for the synthesis of C-dots from inexpensive, natural sources. In this study, we developed a simple method to generate C-dots from inexpensive chicken egg whites through a one-step heating reaction. The size of the generated C-dots was 3.3 ± 0.4 nm, and the quantum yield of the C-dots was as high as ∼43%. The as-prepared C-dots can be used as multicolor labeling agents for bacteria such as Staphylococcus aureus and Escherichia coli. Furthermore, the generated C-dots can be used as Förster resonance energy transfer sensing probes for curcumin, which is an active ingredient of turmeric and medicinal pigment. The feasibility of using the C-dots as selective sensing probes to determine the amount of curcumin from complex turmeric powder and condensed turmeric tablets is also demonstrated.
KW - Bacteria
KW - Carbon dots
KW - Curcumin
KW - Fluorescence
KW - Förster resonance energy transfer (FRET)
UR - http://www.scopus.com/inward/record.url?scp=85018259951&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2017.04.045
DO - 10.1016/j.jcis.2017.04.045
M3 - Article
C2 - 28463765
AN - SCOPUS:85018259951
SN - 0021-9797
VL - 501
SP - 341
EP - 349
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
ER -