Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats

Yu Feng Hu; An Sheng Lee; Shih Lin Chang; Shien Fong Lin; Ching Hui Weng; Hsin Yu Lo; Pei Chun Chou; Yung Nan Tsai; Yen Ling Sung; Chien Chang Chen; Ruey Bing Yang; Yuh Charn Lin; Terry B.J. Kuo; Cheng Han Wu; Jin Dian Liu; Tze Wen Chung; Shih Ann Chen

doi:10.1038/s41551-021-00812-y

Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats

Yu Feng Hu^*, An Sheng Lee, Shih Lin Chang, Shien Fong Lin, Ching Hui Weng, Hsin Yu Lo, Pei Chun Chou, Yung Nan Tsai, Yen Ling Sung, Chien Chang Chen, Ruey Bing Yang, Yuh Charn Lin, Terry B.J. Kuo, Cheng Han Wu, Jin Dian Liu, Tze Wen Chung, Shih Ann Chen

^*此作品的通信作者

研究成果: Article › 同行評審

11 引文斯高帕斯（Scopus）

摘要

Pacemaker cells can be differentiated from stem cells or transdifferentiated from quiescent mature cardiac cells via genetic manipulation. Here we show that the exposure of rat quiescent ventricular cardiomyocytes to a silk-fibroin hydrogel activates the direct conversion of the quiescent cardiomyocytes to pacemaker cardiomyocytes by inducing the ectopic expression of the vascular endothelial cell-adhesion glycoprotein cadherin. The silk-fibroin-induced pacemaker cells exhibited functional and morphological features of genuine sinoatrial-node cardiomyocytes in vitro, and pacemaker cells generated via the injection of silk fibroin in the left ventricles of rats functioned as a surrogate in situ sinoatrial node. Biomaterials with suitable surface structure, mechanics and biochemistry could facilitate the scalable production of biological pacemakers for human use.

原文	English
期刊	Nature Biomedical Engineering
DOIs	https://doi.org/10.1038/s41551-021-00812-y
出版狀態	Accepted/In press - 2021

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10.1038/s41551-021-00812-y

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Hu, Y. F., Lee, A. S., Chang, S. L., Lin, S. F., Weng, C. H., Lo, H. Y., Chou, P. C., Tsai, Y. N., Sung, Y. L., Chen, C. C., Yang, R. B., Lin, Y. C., Kuo, T. B. J., Wu, C. H., Liu, J. D., Chung, T. W., & Chen, S. A. (認可的出版社/出版中). Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-021-00812-y

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title = "Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats",

abstract = "Pacemaker cells can be differentiated from stem cells or transdifferentiated from quiescent mature cardiac cells via genetic manipulation. Here we show that the exposure of rat quiescent ventricular cardiomyocytes to a silk-fibroin hydrogel activates the direct conversion of the quiescent cardiomyocytes to pacemaker cardiomyocytes by inducing the ectopic expression of the vascular endothelial cell-adhesion glycoprotein cadherin. The silk-fibroin-induced pacemaker cells exhibited functional and morphological features of genuine sinoatrial-node cardiomyocytes in vitro, and pacemaker cells generated via the injection of silk fibroin in the left ventricles of rats functioned as a surrogate in situ sinoatrial node. Biomaterials with suitable surface structure, mechanics and biochemistry could facilitate the scalable production of biological pacemakers for human use.",

author = "Hu, {Yu Feng} and Lee, {An Sheng} and Chang, {Shih Lin} and Lin, {Shien Fong} and Weng, {Ching Hui} and Lo, {Hsin Yu} and Chou, {Pei Chun} and Tsai, {Yung Nan} and Sung, {Yen Ling} and Chen, {Chien Chang} and Yang, {Ruey Bing} and Lin, {Yuh Charn} and Kuo, {Terry B.J.} and Wu, {Cheng Han} and Liu, {Jin Dian} and Chung, {Tze Wen} and Chen, {Shih Ann}",

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year = "2021",

doi = "10.1038/s41551-021-00812-y",

language = "English",

journal = "Nature Biomedical Engineering",

issn = "2157-846X",

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TY - JOUR

T1 - Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats

AU - Hu, Yu Feng

AU - Lee, An Sheng

AU - Chang, Shih Lin

AU - Lin, Shien Fong

AU - Weng, Ching Hui

AU - Lo, Hsin Yu

AU - Chou, Pei Chun

AU - Tsai, Yung Nan

AU - Sung, Yen Ling

AU - Chen, Chien Chang

AU - Yang, Ruey Bing

AU - Lin, Yuh Charn

AU - Kuo, Terry B.J.

AU - Wu, Cheng Han

AU - Liu, Jin Dian

AU - Chung, Tze Wen

AU - Chen, Shih Ann

PY - 2021

Y1 - 2021

N2 - Pacemaker cells can be differentiated from stem cells or transdifferentiated from quiescent mature cardiac cells via genetic manipulation. Here we show that the exposure of rat quiescent ventricular cardiomyocytes to a silk-fibroin hydrogel activates the direct conversion of the quiescent cardiomyocytes to pacemaker cardiomyocytes by inducing the ectopic expression of the vascular endothelial cell-adhesion glycoprotein cadherin. The silk-fibroin-induced pacemaker cells exhibited functional and morphological features of genuine sinoatrial-node cardiomyocytes in vitro, and pacemaker cells generated via the injection of silk fibroin in the left ventricles of rats functioned as a surrogate in situ sinoatrial node. Biomaterials with suitable surface structure, mechanics and biochemistry could facilitate the scalable production of biological pacemakers for human use.

AB - Pacemaker cells can be differentiated from stem cells or transdifferentiated from quiescent mature cardiac cells via genetic manipulation. Here we show that the exposure of rat quiescent ventricular cardiomyocytes to a silk-fibroin hydrogel activates the direct conversion of the quiescent cardiomyocytes to pacemaker cardiomyocytes by inducing the ectopic expression of the vascular endothelial cell-adhesion glycoprotein cadherin. The silk-fibroin-induced pacemaker cells exhibited functional and morphological features of genuine sinoatrial-node cardiomyocytes in vitro, and pacemaker cells generated via the injection of silk fibroin in the left ventricles of rats functioned as a surrogate in situ sinoatrial node. Biomaterials with suitable surface structure, mechanics and biochemistry could facilitate the scalable production of biological pacemakers for human use.

UR - http://www.scopus.com/inward/record.url?scp=85119699930&partnerID=8YFLogxK

U2 - 10.1038/s41551-021-00812-y

DO - 10.1038/s41551-021-00812-y

M3 - Article

C2 - 34811487

AN - SCOPUS:85119699930

SN - 2157-846X

JO - Nature Biomedical Engineering

JF - Nature Biomedical Engineering

ER -

Biomaterial-induced conversion of quiescent cardiomyocytes into pacemaker cells in rats

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