Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer

Jiantong Dong; Ryan Y. Zhang; Na Sun; Matthew Smalley; Zipeng Wu; Anqi Zhou; Shih Jie Chou; Yu Jen Jan; Peng Yang; Lirong Bao; Dongping Qi; Xinghong Tang; Patrick Tseng; Yue Hua; Dianwen Xu; Rueihung Kao; Meng Meng; Xirun Zheng; Ying Liu; Tatyana Vagner; Xiaoshu Chai; Dongjing Zhou; Mengyuan Li; Shih Hwa Chiou; Guangjuan Zheng; Dolores Di Vizio; Vatche G. Agopian; Edwin Posadas; Steven J. Jonas; Shin Pon Ju; Paul S. Weiss; Meiping Zhao; Hsian Rong Tseng; Yazhen Zhu

doi:10.1021/acsami.9b01406

Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer

Jiantong Dong, Ryan Y. Zhang, Na Sun, Matthew Smalley, Zipeng Wu, Anqi Zhou, Shih Jie Chou, Yu Jen Jan, Peng Yang, Lirong Bao, Dongping Qi, Xinghong Tang, Patrick Tseng, Yue Hua, Dianwen Xu, Rueihung Kao, Meng Meng, Xirun Zheng, Ying Liu, Tatyana VagnerXiaoshu Chai, Dongjing Zhou, Mengyuan Li, Shih Hwa Chiou, Guangjuan Zheng, Dolores Di Vizio, Vatche G. Agopian, Edwin Posadas, Steven J. Jonas, Shin Pon Ju^*, Paul S. Weiss, Meiping Zhao, Hsian Rong Tseng, Yazhen Zhu

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研究成果: Article › 同行評審

51 引文斯高帕斯（Scopus）

摘要

Tumor-derived extracellular vesicles (EVs) present in bodily fluids are emerging liquid biopsy markers for non-invasive cancer diagnosis and treatment monitoring. Because the majority of EVs in circulation are not of tumor origin, it is critical to develop new platforms capable of enriching tumor-derived EVs from the blood. Herein, we introduce a biostructure-inspired NanoVilli Chip, capable of highly efficient and reproducible immunoaffinity capture of tumor-derived EVs from blood plasma samples. Anti-EpCAM-grafted silicon nanowire arrays were engineered to mimic the distinctive structures of intestinal microvilli, dramatically increasing surface area and enhancing tumor-derived EV capture. RNA in the captured EVs can be recovered for downstream molecular analyses by reverse transcription Droplet Digital PCR. We demonstrate that this assay can be applied to monitor the dynamic changes of ROS1 rearrangements and epidermal growth factor receptor T790M mutations that predict treatment responses and disease progression in non-small cell lung cancer patients.

原文	English
頁（從 - 到）	13973-13983
頁數	11
期刊	ACS Applied Materials and Interfaces
卷	11
發行號	15
DOIs	https://doi.org/10.1021/acsami.9b01406
出版狀態	Published - 17 4月 2019

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10.1021/acsami.9b01406

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Dong, J., Zhang, R. Y., Sun, N., Smalley, M., Wu, Z., Zhou, A., Chou, S. J., Jan, Y. J., Yang, P., Bao, L., Qi, D., Tang, X., Tseng, P., Hua, Y., Xu, D., Kao, R., Meng, M., Zheng, X., Liu, Y., ... Zhu, Y. (2019). Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer. ACS Applied Materials and Interfaces, 11(15), 13973-13983. https://doi.org/10.1021/acsami.9b01406

@article{0be04113571141b88c10c53797851698,

title = "Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer",

abstract = "Tumor-derived extracellular vesicles (EVs) present in bodily fluids are emerging liquid biopsy markers for non-invasive cancer diagnosis and treatment monitoring. Because the majority of EVs in circulation are not of tumor origin, it is critical to develop new platforms capable of enriching tumor-derived EVs from the blood. Herein, we introduce a biostructure-inspired NanoVilli Chip, capable of highly efficient and reproducible immunoaffinity capture of tumor-derived EVs from blood plasma samples. Anti-EpCAM-grafted silicon nanowire arrays were engineered to mimic the distinctive structures of intestinal microvilli, dramatically increasing surface area and enhancing tumor-derived EV capture. RNA in the captured EVs can be recovered for downstream molecular analyses by reverse transcription Droplet Digital PCR. We demonstrate that this assay can be applied to monitor the dynamic changes of ROS1 rearrangements and epidermal growth factor receptor T790M mutations that predict treatment responses and disease progression in non-small cell lung cancer patients.",

keywords = "EGFR T790M mutation, ROS1 rearrangements, extracellular vesicles, microfluidics, nanosubstrates, non-small cell lung cancer",

author = "Jiantong Dong and Zhang, {Ryan Y.} and Na Sun and Matthew Smalley and Zipeng Wu and Anqi Zhou and Chou, {Shih Jie} and Jan, {Yu Jen} and Peng Yang and Lirong Bao and Dongping Qi and Xinghong Tang and Patrick Tseng and Yue Hua and Dianwen Xu and Rueihung Kao and Meng Meng and Xirun Zheng and Ying Liu and Tatyana Vagner and Xiaoshu Chai and Dongjing Zhou and Mengyuan Li and Chiou, {Shih Hwa} and Guangjuan Zheng and {Di Vizio}, Dolores and Agopian, {Vatche G.} and Edwin Posadas and Jonas, {Steven J.} and Ju, {Shin Pon} and Weiss, {Paul S.} and Meiping Zhao and Tseng, {Hsian Rong} and Yazhen Zhu",

year = "2019",

month = apr,

day = "17",

doi = "10.1021/acsami.9b01406",

language = "English",

volume = "11",

pages = "13973--13983",

journal = "ACS Applied Materials and Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society",

number = "15",

}

Dong, J, Zhang, RY, Sun, N, Smalley, M, Wu, Z, Zhou, A, Chou, SJ, Jan, YJ, Yang, P, Bao, L, Qi, D, Tang, X, Tseng, P, Hua, Y, Xu, D, Kao, R, Meng, M, Zheng, X, Liu, Y, Vagner, T, Chai, X, Zhou, D, Li, M, Chiou, SH, Zheng, G, Di Vizio, D, Agopian, VG, Posadas, E, Jonas, SJ, Ju, SP, Weiss, PS, Zhao, M, Tseng, HR & Zhu, Y 2019, 'Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer', ACS Applied Materials and Interfaces, 卷 11, 編號 15, 頁 13973-13983. https://doi.org/10.1021/acsami.9b01406

TY - JOUR

T1 - Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles

T2 - Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer

AU - Dong, Jiantong

AU - Zhang, Ryan Y.

AU - Sun, Na

AU - Smalley, Matthew

AU - Wu, Zipeng

AU - Zhou, Anqi

AU - Chou, Shih Jie

AU - Jan, Yu Jen

AU - Yang, Peng

AU - Bao, Lirong

AU - Qi, Dongping

AU - Tang, Xinghong

AU - Tseng, Patrick

AU - Hua, Yue

AU - Xu, Dianwen

AU - Kao, Rueihung

AU - Meng, Meng

AU - Zheng, Xirun

AU - Liu, Ying

AU - Vagner, Tatyana

AU - Chai, Xiaoshu

AU - Zhou, Dongjing

AU - Li, Mengyuan

AU - Chiou, Shih Hwa

AU - Zheng, Guangjuan

AU - Di Vizio, Dolores

AU - Agopian, Vatche G.

AU - Posadas, Edwin

AU - Jonas, Steven J.

AU - Ju, Shin Pon

AU - Weiss, Paul S.

AU - Zhao, Meiping

AU - Tseng, Hsian Rong

AU - Zhu, Yazhen

PY - 2019/4/17

Y1 - 2019/4/17

N2 - Tumor-derived extracellular vesicles (EVs) present in bodily fluids are emerging liquid biopsy markers for non-invasive cancer diagnosis and treatment monitoring. Because the majority of EVs in circulation are not of tumor origin, it is critical to develop new platforms capable of enriching tumor-derived EVs from the blood. Herein, we introduce a biostructure-inspired NanoVilli Chip, capable of highly efficient and reproducible immunoaffinity capture of tumor-derived EVs from blood plasma samples. Anti-EpCAM-grafted silicon nanowire arrays were engineered to mimic the distinctive structures of intestinal microvilli, dramatically increasing surface area and enhancing tumor-derived EV capture. RNA in the captured EVs can be recovered for downstream molecular analyses by reverse transcription Droplet Digital PCR. We demonstrate that this assay can be applied to monitor the dynamic changes of ROS1 rearrangements and epidermal growth factor receptor T790M mutations that predict treatment responses and disease progression in non-small cell lung cancer patients.

AB - Tumor-derived extracellular vesicles (EVs) present in bodily fluids are emerging liquid biopsy markers for non-invasive cancer diagnosis and treatment monitoring. Because the majority of EVs in circulation are not of tumor origin, it is critical to develop new platforms capable of enriching tumor-derived EVs from the blood. Herein, we introduce a biostructure-inspired NanoVilli Chip, capable of highly efficient and reproducible immunoaffinity capture of tumor-derived EVs from blood plasma samples. Anti-EpCAM-grafted silicon nanowire arrays were engineered to mimic the distinctive structures of intestinal microvilli, dramatically increasing surface area and enhancing tumor-derived EV capture. RNA in the captured EVs can be recovered for downstream molecular analyses by reverse transcription Droplet Digital PCR. We demonstrate that this assay can be applied to monitor the dynamic changes of ROS1 rearrangements and epidermal growth factor receptor T790M mutations that predict treatment responses and disease progression in non-small cell lung cancer patients.

KW - EGFR T790M mutation

KW - ROS1 rearrangements

KW - extracellular vesicles

KW - microfluidics

KW - nanosubstrates

KW - non-small cell lung cancer

UR - http://www.scopus.com/inward/record.url?scp=85064136995&partnerID=8YFLogxK

U2 - 10.1021/acsami.9b01406

DO - 10.1021/acsami.9b01406

M3 - Article

C2 - 30892008

AN - SCOPUS:85064136995

SN - 1944-8244

VL - 11

SP - 13973

EP - 13983

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 15

ER -

Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer

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