Behavior training reverses asymmetry in hippocampal transcriptome of the Cav3.2 knockout mice

Ni Chun Chung, Ying Hsueh Huang, Chuan Hsiung Chang, James C. Liao, Chih Hsien Yang, Chien Chang Chen, Ingrid Y. Liu

研究成果: Article同行評審

7 引文 斯高帕斯(Scopus)

摘要

Homozygous Cav3.2 knockout mice, which are defective in the pore-forming subunit of a low voltage activated T-type calcium channel, have been documented to show impaired maintenance of late-phase long-term potentiation (L-LTP) and defective retrieval of contextassociated fear memory. To investigate the role of Cav3.2 in global gene expression, we performed a microarray transcriptome study on the hippocampi of the Cav3.2-/- mice and their wild-type littermates, either naïve (untrained) or trace fear conditioned. We found a significant left-right asymmetric effect on the hippocampal transcriptome caused by the Cav3.2 knockout. Between the naive Cav3.2-/- and the naive wild-type mice, 3522 differentially expressed genes (DEGs) were found in the left hippocampus, but only 4 DEGs were found in the right hippocampus. Remarkably, the effect of Cav3.2 knockout was partially reversed by trace fear conditioning. The number of DEGs in the left hippocampus was reduced to 6 in the Cav3.2 knockout mice after trace fear conditioning, compared with the wild-type naïve mice. To our knowledge, these results demonstrate for the first time the asymmetric effects of the Cav3.2 and its partial reversal by behavior training on the hippocampal transcriptome.

原文English
文章編號e0118832
期刊PLoS ONE
10
發行號3
DOIs
出版狀態Published - 13 3月 2015

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