Bead-based digital microfluidic immunoassay for IL-1β detection in embryo culture medium

Hsueh Yang Tseng, Yi Ting Jiang, Cheng Yeh Huang, Meng Shiue Lee, Wen-Syang Hsu, Da Jeng Yao

研究成果: Conference contribution同行評審

摘要

One of the challenges in IVF treatment is to improve the pregnancy rate of selective single embryo implantation. The concentration of IL-1β in embryo culture medium may help to predict pregnancy rate. Clinically, each embryo can only get small amount of sample size. However, the traditional immunoassay cannot detect biomarkers in such small sample sizes. Therefore, we combined immunoassay with the bead-based digital microfluidic chip. The chip has two advantages, low sample requirement and low detection limit. We established the mouse IL-1β calibration curve, confirm the Human Tubal Fluid (HTF) does not influence the test result and demonstrate that mouse IL-1β in clinical mouse embryo culture medium is positively correlate to cultured days. This device has a great potential for increasing IVF pregnancy rates.

原文English
主出版物標題2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
發行者Institute of Electrical and Electronics Engineers Inc.
頁面295-298
頁數4
ISBN(電子)9781509030590
DOIs
出版狀態Published - 25 8月 2017
事件12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, 美國
持續時間: 9 4月 201712 4月 2017

出版系列

名字2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Conference

Conference12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
國家/地區美國
城市Los Angeles
期間9/04/1712/04/17

指紋

深入研究「Bead-based digital microfluidic immunoassay for IL-1β detection in embryo culture medium」主題。共同形成了獨特的指紋。

引用此