Assembling an ion channel: ORF 3a from SARS-CoV

Tze Hsiang Chien; Ya Ling Chiang; Chin Pei Chen; Petra Henklein; Karen Hänel; Ing Shouh Hwang; Dieter Willbold; Wolfgang B. Fischer

doi:10.1002/bip.22230

Assembling an ion channel: ORF 3a from SARS-CoV

Tze Hsiang Chien, Ya Ling Chiang, Chin Pei Chen, Petra Henklein, Karen Hänel, Ing Shouh Hwang, Dieter Willbold, Wolfgang B. Fischer^*

^*此作品的通信作者

生醫光電研究所

研究成果: Article › 同行評審

12 引文斯高帕斯（Scopus）

摘要

Protein 3a is a 274 amino acid polytopic channel protein with three putative transmembrane domains (TMDs) encoded by severe acute respiratory syndrome corona virus (SARS-CoV). Synthetic peptides corresponding to each of its three individual transmembrane domains (TMDs) are reconstituted into artificial lipid bilayers. Only TMD2 and TMD3 induce channel activity. Reconstitution of the peptides as TMD1 + TMD3 as well as TMD2 + TMD3 in a 1: 1 mixture induces membrane activity for both mixtures. In a 1: 1: 1 mixture, channel like behavior is almost restored. Expression of full length 3a and reconstitution into artificial lipid bilayers reveal a weak cation selective (P_K ≈ 2 P_Cl) rectifying channel. In the presence of nonphysiological concentration of Ca-ions the channel develops channel activity.

原文	English
頁（從 - 到）	628-635
頁數	8
期刊	Biopolymers
卷	99
發行號	9
DOIs	https://doi.org/10.1002/bip.22230
出版狀態	Published - 9月 2013

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abstract = "Protein 3a is a 274 amino acid polytopic channel protein with three putative transmembrane domains (TMDs) encoded by severe acute respiratory syndrome corona virus (SARS-CoV). Synthetic peptides corresponding to each of its three individual transmembrane domains (TMDs) are reconstituted into artificial lipid bilayers. Only TMD2 and TMD3 induce channel activity. Reconstitution of the peptides as TMD1 + TMD3 as well as TMD2 + TMD3 in a 1: 1 mixture induces membrane activity for both mixtures. In a 1: 1: 1 mixture, channel like behavior is almost restored. Expression of full length 3a and reconstitution into artificial lipid bilayers reveal a weak cation selective (PK ≈ 2 PCl) rectifying channel. In the presence of nonphysiological concentration of Ca-ions the channel develops channel activity.",

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AU - Chien, Tze Hsiang

AU - Chiang, Ya Ling

AU - Chen, Chin Pei

AU - Henklein, Petra

AU - Hänel, Karen

AU - Hwang, Ing Shouh

AU - Willbold, Dieter

AU - Fischer, Wolfgang B.

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KW - ORF3a of SARS-CoV

KW - bilayer recordings

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KW - self-assembly

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Assembling an ion channel: ORF 3a from SARS-CoV

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