TY - GEN
T1 - Application of Metal-Enhanced Fluorescence technology in evanescent wave fluorescent biosensor
AU - Su, Yu Zheng
AU - Hung, Min Wei
AU - Wu, Wen Hong
AU - Huang, Kuo Cheng
AU - Chiang, Huihua Kenny
PY - 2010
Y1 - 2010
N2 - Evanescent wave fluorescent sensors have developed for 30 years. Nonetheless, the technology continues to evolve with new breakthroughs in optics, biochemistry, and chemical engineering. Recently, there have been explosive developments in the Metal-Enhanced Fluorescence (MEF) technology to favorably modify the spectral properties and to alleviate photo-physical constraints. We report the development of core-shell nanoparticles with the silver core and SiO2 shell for potential applications in fiber optic biosensor. The fluorescence intensity of the fluorescence probe (Fluorescein isothiocyanate, FITC) doped core-shell nanoparticles was approximately 50-fold higher than that without core-shell nanoparticles doping. In addition, four different kinds of organic solvents are used in this study and found to have influence on the fluorescence intensity from 1.3 to over 100 times. We conclude that core-shell nanoparticles have capability to enhance the fiber optic sensing through amplifying fluorescence intensity and expect that their potential applications are used in a variety of biological applications.
AB - Evanescent wave fluorescent sensors have developed for 30 years. Nonetheless, the technology continues to evolve with new breakthroughs in optics, biochemistry, and chemical engineering. Recently, there have been explosive developments in the Metal-Enhanced Fluorescence (MEF) technology to favorably modify the spectral properties and to alleviate photo-physical constraints. We report the development of core-shell nanoparticles with the silver core and SiO2 shell for potential applications in fiber optic biosensor. The fluorescence intensity of the fluorescence probe (Fluorescein isothiocyanate, FITC) doped core-shell nanoparticles was approximately 50-fold higher than that without core-shell nanoparticles doping. In addition, four different kinds of organic solvents are used in this study and found to have influence on the fluorescence intensity from 1.3 to over 100 times. We conclude that core-shell nanoparticles have capability to enhance the fiber optic sensing through amplifying fluorescence intensity and expect that their potential applications are used in a variety of biological applications.
KW - Biosensor
KW - Fluorescence
KW - Metalenhanced fluorescence
KW - Nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=77957842091&partnerID=8YFLogxK
U2 - 10.1109/IMTC.2010.5488270
DO - 10.1109/IMTC.2010.5488270
M3 - Conference contribution
AN - SCOPUS:77957842091
SN - 9781424428335
T3 - 2010 IEEE International Instrumentation and Measurement Technology Conference, I2MTC 2010 - Proceedings
SP - 574
EP - 578
BT - 2010 IEEE International Instrumentation and Measurement Technology Conference, I2MTC 2010 - Proceedings
T2 - 2010 IEEE International Instrumentation and Measurement Technology Conference, I2MTC 2010
Y2 - 3 May 2010 through 6 May 2010
ER -