Application of high-resolution single-channel recording to functional studies of cystic fibrosis mutants.

Zhiwei Cai*, Yoshiro Sohma, Silvia G. Bompadre, David N. Sheppard, Tzyh Chang Hwang

*此作品的通信作者

研究成果: Article同行評審

10 引文 斯高帕斯(Scopus)

摘要

The patch-clamp technique is a powerful and versatile method to investigate the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel, its malfunction in disease and modulation by small molecules. Here, we discuss how the molecular behaviour of CFTR is investigated using high-resolution single-channel recording and kinetic analyses of channel gating. We review methods used to quantify how cystic fibrosis (CF) mutants perturb the biophysical properties and regulation of CFTR. By explaining the relationship between macroscopic and single-channel currents, we demonstrate how single-channel data provide molecular explanations for changes in CFTR-mediated transepithelial ion transport elicited by CF mutants.

原文English
頁(從 - 到)419-441
頁數23
期刊Methods in molecular biology (Clifton, N.J.)
741
DOIs
出版狀態Published - 2011

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