Antioxidant effect and active components of litchi (Litchi chinensis Sonn.) flower

Deng Jye Yang, Yan Zin Chang, Yi Chen Chen, Shih Chuan Liu, Chuan Hsiang Hsu, Jau Tien Lin*


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29 引文 斯高帕斯(Scopus)


The effects of scavenging 2, 2-diphenyl-2-picrylhydrazyl hydrate (DPPH) radicals and inhibiting low-density lipoprotein (LDL) oxidation, and phenolic quantities were used for the activity-guided separation to identify the effective components of litchi flower. The acetone extract of the flower with notable antioxidant capacities was suspended in water and sequentially partitioned with n-hexane, ethyl acetate (EA) and n-butanol. The EA partition with the highest phenolic levels and antioxidant capacities was subjected to silica gel column chromatography. Thirteen fractions (Fr. 1-13) were collected; Fr. 10-12 with higher phenolic levels and antioxidant effects were applied to Sephadex LH-20 column chromatography. Each fraction was further separated into three sub-fractions and the second ones (Fr. 10-II, 11-II, and 12-II) were the best, which two major compounds could be isolated by semi-preparative high performance liquid chromatography (HPLC). Through Mass (MS) and Nuclear Magnetic Resonance (NMR) measurements, they could be identified as (-)-epicatechin and proanthocyanidin A2. Their contents in the litchi flower were 5.52 and 11.12. mg/g of dry weight, respectively. The study was the first time to reveal the effective antioxidant components of litchi flower.

頁(從 - 到)3056-3061
期刊Food and Chemical Toxicology
出版狀態Published - 9月 2012


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