TY - JOUR
T1 - Antioxidant, anti-proliferative and cyclooxygenase-2 inhibitory activities of ethanolic extracts from lemon balm (Melissa officinalis L.) leaves
AU - Lin, Jau Tien
AU - Chen, Yi Chen
AU - Lee, Ying Chung
AU - Rolis Hou, Chien Wei
AU - Chen, Fong Lin
AU - Yang, Deng Jye
N1 - Funding Information:
We thank Dr. Long-Zen Chang at Taichung District Agricultural Improvement Station for generously providing lemon balm samples. This work was supported by Chun Shan Medical University, Taichung, Taiwan (project no. CSMU-96-OM-B-005 and CSMU-96-OM-A-088).
PY - 2012/11
Y1 - 2012/11
N2 - Antioxidant, anti-proliferative and cyclooxygenase-2 (COX-2) inhibitory activities of ethanolic extracts from freeze (EF) and hot air (EH) dried lemon balm (. Melissa officinalis L.) leaves were evaluated. Phytochemical contents in the extracts were also determined. Hot air drying significantly lowered phytochemical contents and biological activities of the extract of lemon balm leaves as compared with freeze drying. EF had higher levels of phenols, phenolic acids, flavonoids, proanthocyanidins, ascorbic acid and . γ-tocopherol than EH. Rosmarinic acid was the major compound and hesperetin was the highest level of flavonoid in the extracts. EF also presented higher antioxidant (. β-carotene bleaching inhibition, scavenging 2, 2-diphenyl-1-picrylhydrazyl radicals, reducing power and Trolox equivalent antioxidant capacity), anti-proliferative (proliferative inhibition for Hep G2, KB and TSGH 9201 human cancer cells) and COX-2 (induced by 12-. O-teradecanoylphorbol-13-acetate in KB cells) suppressing activities than EH. The ethanolic extracts of lemon balm leaves, especially EF, may have the potential for cancer chemoprevention.
AB - Antioxidant, anti-proliferative and cyclooxygenase-2 (COX-2) inhibitory activities of ethanolic extracts from freeze (EF) and hot air (EH) dried lemon balm (. Melissa officinalis L.) leaves were evaluated. Phytochemical contents in the extracts were also determined. Hot air drying significantly lowered phytochemical contents and biological activities of the extract of lemon balm leaves as compared with freeze drying. EF had higher levels of phenols, phenolic acids, flavonoids, proanthocyanidins, ascorbic acid and . γ-tocopherol than EH. Rosmarinic acid was the major compound and hesperetin was the highest level of flavonoid in the extracts. EF also presented higher antioxidant (. β-carotene bleaching inhibition, scavenging 2, 2-diphenyl-1-picrylhydrazyl radicals, reducing power and Trolox equivalent antioxidant capacity), anti-proliferative (proliferative inhibition for Hep G2, KB and TSGH 9201 human cancer cells) and COX-2 (induced by 12-. O-teradecanoylphorbol-13-acetate in KB cells) suppressing activities than EH. The ethanolic extracts of lemon balm leaves, especially EF, may have the potential for cancer chemoprevention.
KW - Anti-proliferative
KW - Antioxidant
KW - Cyclooxygenase-2 (COX-2)
KW - Drying
KW - Melissa officinalis L.
UR - http://www.scopus.com/inward/record.url?scp=84862690600&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2012.04.009
DO - 10.1016/j.lwt.2012.04.009
M3 - Article
AN - SCOPUS:84862690600
SN - 0023-6438
VL - 49
SP - 1
EP - 7
JO - LWT - Food Science and Technology
JF - LWT - Food Science and Technology
IS - 1
ER -
