Anaerobic biotransformation of fluorene and phenanthrene by sulfate-reducing bacteria and identification of biotransformation pathway

Jen Chieh Tsai, Mathava Kumar, Jih-Gaw Lin*

*此作品的通信作者

研究成果: Article同行評審

75 引文 斯高帕斯(Scopus)

摘要

In the present study, anaerobic biotransformation of fluorene and phenanthrene by sulfate-reducing bacteria (SRB) was investigated and biotransformation pathways were proposed. SRB was enriched from anaerobic swine wastewater sludge and its abundance was determined by the fluorescence in situ hybridization (FISH) technique. Batch anaerobic biotransformation studies were conducted with fluorene (5 mg L-1), phenanthrene (5 mg L-1) and a mixture of the two (10 mg L-1). After 21 d of incubation, 88% of fluorene and 65% of phenanthrene were biotransformed by SRB. In contrast to previous studies, a decrease in biotransformation efficiency was observed in the presence of both fluorene and phenanthrene. Throughout the study, sulfate reduction was coupled with biotransformation of fluorene and phenanthrene. However, no increase in bacterial cell density was observed in the presence of an inhibitor, i.e. molybdate. Identification of metabolites by gas chromatography-mass spectrometry (GC-MS) revealed that fluorene and phenanthrene were biotransformed through a sequence of hydration and hydrolysis reactions followed by decarboxylation with the formation of p-cresol (only in the phenanthrene system) and phenol. The metabolites identified suggest novel biotransformation pathways of fluorene and phenenthrene.

原文English
頁(從 - 到)847-855
頁數9
期刊Journal of Hazardous Materials
164
發行號2-3
DOIs
出版狀態Published - 30 5月 2009

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