An integrated microfluidic system for diagnosis of quinolones resistance of helicobacter pylori

Chih Yu Chao, Chih Hung Wang, Yu Jui Che, Cheng Yen Kao, Jiunn Jong Wu, Gwo Bin Lee

研究成果: Conference contribution同行評審

2 引文 斯高帕斯(Scopus)

摘要

Helicobacter pylori (H. pylori) is a bacterium which can colonize the stomach mucosa and therefore play a crucial role in gastric diseases. Triple therapy treatment consisting of two antibiotics and a proton pump inhibitor has been routinely taken to eradicate H. pylori. Recently, some point mutations were found in gyrase genes against Quinolones. The highly frequent mutation sites were indicated as single amino acid substitution discovered in gyrase A subunit. The epsilometer test has been commonly used to confirm the antibiotic resistance after triple therapy treatment. However, the method is time-consuming and false negative results with trace amounts of H. pylori could be easily induced. Alternatively, conventional molecular diagnostic techniques such as polymer chain reaction (PCR) could be used to confirm the antibiotic resistance of H. pylori. However, this diagnostic process is relatively labor-intensive and requires expensive and bulky apparatus. In this study a new method was therefore developed to perform molecular diagnostic techniques of SNP-PCR on an integrated microfluidic system to detect the Quinolones resistance of H. pylori.

原文English
主出版物標題MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
發行者Institute of Electrical and Electronics Engineers Inc.
頁面821-824
頁數4
ISBN(列印)9781479935086
DOIs
出版狀態Published - 2014
事件27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States
持續時間: 26 1月 201430 1月 2014

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(列印)1084-6999

Conference

Conference27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
國家/地區United States
城市San Francisco, CA
期間26/01/1430/01/14

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