@inproceedings{6db60870a16d4774a3ccab5cfb37f1d2,
title = "An integrated microfluidic system for detection of live bacteria by using ethidium monoazide and loop-mediated isothermal amplification",
abstract = "Diagnosis of periprosthetic joint infection (PJI) is a clinical challenge for implant surgery. In this work, a new integrated microfluidic system by using ethidium monoazide (EMA) treatment and loop-mediated isothermal amplification (LAMP) was reported for fast diagnosis of PJI. It is the first time that EMA and LAMP approaches are used for detection of {"}live{"} bacteria in joint fluids. Furthermore, this whole diagnostic process including sample pretreatment, DNA amplification and optical detection was automatically performed on the integrated microfluidic chip by using a compact custom-made system. Therefore, this detection method of PJI is potentially feasible for on-site diagnosis.",
keywords = "Ethidium monoazide (EMA), Loop-mediated isothermal amplification (LAMP), Microfluidics, Periprosthetic joint infection (PJI)",
author = "Chen, {Shu Ling} and Chang, {Wen Hsin} and Wang, {Chih Hung} and Wu, {Jiunn Jong} and Lee, {Mel S.} and Lee, {Gwo Bin}",
note = "Publisher Copyright: {\textcopyright} 15CBMS-0001.; 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 ; Conference date: 25-10-2015 Through 29-10-2015",
year = "2015",
month = oct,
language = "English",
series = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "317--319",
booktitle = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}